Cargando…

Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer

Imaging modalities using tumor-directed monoclonal antibodies may be of value to improve the pre- and intraoperative detection and resection of lymph node (LN) metastatic disease in penile squamous cell carcinoma (PSCC). We investigated the expression of prostate-specific membrane antigen (PSMA), va...

Descripción completa

Detalles Bibliográficos
Autores principales: van der Fels, Christa A. M., Palthe, Selma, Buikema, Henk, van den Heuvel, Marius C., Leliveld, Annemarie, de Jong, Igle Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555672/
https://www.ncbi.nlm.nih.gov/pubmed/32942549
http://dx.doi.org/10.3390/diagnostics10090694
_version_ 1783594062040793088
author van der Fels, Christa A. M.
Palthe, Selma
Buikema, Henk
van den Heuvel, Marius C.
Leliveld, Annemarie
de Jong, Igle Jan
author_facet van der Fels, Christa A. M.
Palthe, Selma
Buikema, Henk
van den Heuvel, Marius C.
Leliveld, Annemarie
de Jong, Igle Jan
author_sort van der Fels, Christa A. M.
collection PubMed
description Imaging modalities using tumor-directed monoclonal antibodies may be of value to improve the pre- and intraoperative detection and resection of lymph node (LN) metastatic disease in penile squamous cell carcinoma (PSCC). We investigated the expression of prostate-specific membrane antigen (PSMA), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR) and epithelial cell adhesion molecule (EpCAM) to analyze their potency for diagnostic applications. Antigen expression was determined in primary tumors and LNs with and without metastases of 22 patients with PSCC. The total immunostaining score (TIS, 0–12) was determined as the product of a proportion score (PS, 0–4) and an intensity score (IS, 0–3). EGFR and VEGF expression were high in primary tumor (median TIS 8) and LN metastases (median TIS 6 and 8, respectively). No EGFR expression was seen in LNs without metastases. However, LNs without metastases did show VEGF expression (median TIS 6). No EpCAM or PSMA expression was seen in PSCC. This study shows that VEGF and EGFR expression is moderate to high in LN metastases of PSCC. Both VEGF and EGFR warrant further clinical evaluation to determine their value as a target for pre- and intraoperative imaging modalities in the detection of LN metastases in PSCC.
format Online
Article
Text
id pubmed-7555672
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75556722020-10-19 Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer van der Fels, Christa A. M. Palthe, Selma Buikema, Henk van den Heuvel, Marius C. Leliveld, Annemarie de Jong, Igle Jan Diagnostics (Basel) Article Imaging modalities using tumor-directed monoclonal antibodies may be of value to improve the pre- and intraoperative detection and resection of lymph node (LN) metastatic disease in penile squamous cell carcinoma (PSCC). We investigated the expression of prostate-specific membrane antigen (PSMA), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR) and epithelial cell adhesion molecule (EpCAM) to analyze their potency for diagnostic applications. Antigen expression was determined in primary tumors and LNs with and without metastases of 22 patients with PSCC. The total immunostaining score (TIS, 0–12) was determined as the product of a proportion score (PS, 0–4) and an intensity score (IS, 0–3). EGFR and VEGF expression were high in primary tumor (median TIS 8) and LN metastases (median TIS 6 and 8, respectively). No EGFR expression was seen in LNs without metastases. However, LNs without metastases did show VEGF expression (median TIS 6). No EpCAM or PSMA expression was seen in PSCC. This study shows that VEGF and EGFR expression is moderate to high in LN metastases of PSCC. Both VEGF and EGFR warrant further clinical evaluation to determine their value as a target for pre- and intraoperative imaging modalities in the detection of LN metastases in PSCC. MDPI 2020-09-15 /pmc/articles/PMC7555672/ /pubmed/32942549 http://dx.doi.org/10.3390/diagnostics10090694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van der Fels, Christa A. M.
Palthe, Selma
Buikema, Henk
van den Heuvel, Marius C.
Leliveld, Annemarie
de Jong, Igle Jan
Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title_full Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title_fullStr Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title_full_unstemmed Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title_short Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer
title_sort potential receptors for targeted imaging of lymph node metastases in penile cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555672/
https://www.ncbi.nlm.nih.gov/pubmed/32942549
http://dx.doi.org/10.3390/diagnostics10090694
work_keys_str_mv AT vanderfelschristaam potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer
AT paltheselma potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer
AT buikemahenk potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer
AT vandenheuvelmariusc potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer
AT leliveldannemarie potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer
AT dejongiglejan potentialreceptorsfortargetedimagingoflymphnodemetastasesinpenilecancer