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Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators

Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogeneti...

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Detalles Bibliográficos
Autores principales: Lu, Xiaocen, Shen, Yi, Campbell, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555876/
https://www.ncbi.nlm.nih.gov/pubmed/32906617
http://dx.doi.org/10.3390/ijms21186522
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author Lu, Xiaocen
Shen, Yi
Campbell, Robert E.
author_facet Lu, Xiaocen
Shen, Yi
Campbell, Robert E.
author_sort Lu, Xiaocen
collection PubMed
description Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl).
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spelling pubmed-75558762020-10-19 Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators Lu, Xiaocen Shen, Yi Campbell, Robert E. Int J Mol Sci Review Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl). MDPI 2020-09-07 /pmc/articles/PMC7555876/ /pubmed/32906617 http://dx.doi.org/10.3390/ijms21186522 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lu, Xiaocen
Shen, Yi
Campbell, Robert E.
Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title_full Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title_fullStr Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title_full_unstemmed Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title_short Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
title_sort engineering photosensory modules of non-opsin-based optogenetic actuators
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555876/
https://www.ncbi.nlm.nih.gov/pubmed/32906617
http://dx.doi.org/10.3390/ijms21186522
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