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TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays

Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve...

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Autores principales: Panahipour, Layla, Kargarpour, Zahra, Luza, Bernadette, Lee, Jung-Seok, Gruber, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555929/
https://www.ncbi.nlm.nih.gov/pubmed/32927851
http://dx.doi.org/10.3390/ijms21186636
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author Panahipour, Layla
Kargarpour, Zahra
Luza, Bernadette
Lee, Jung-Seok
Gruber, Reinhard
author_facet Panahipour, Layla
Kargarpour, Zahra
Luza, Bernadette
Lee, Jung-Seok
Gruber, Reinhard
author_sort Panahipour, Layla
collection PubMed
description Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration.
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spelling pubmed-75559292020-10-19 TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays Panahipour, Layla Kargarpour, Zahra Luza, Bernadette Lee, Jung-Seok Gruber, Reinhard Int J Mol Sci Article Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration. MDPI 2020-09-10 /pmc/articles/PMC7555929/ /pubmed/32927851 http://dx.doi.org/10.3390/ijms21186636 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panahipour, Layla
Kargarpour, Zahra
Luza, Bernadette
Lee, Jung-Seok
Gruber, Reinhard
TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title_full TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title_fullStr TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title_full_unstemmed TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title_short TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays
title_sort tgf-β activity related to the use of collagen membranes: in vitro bioassays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555929/
https://www.ncbi.nlm.nih.gov/pubmed/32927851
http://dx.doi.org/10.3390/ijms21186636
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