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Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich envi...

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Autores principales: Bujak, Katarzyna, Decewicz, Przemyslaw, Kaminski, Jerzy, Radlinska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556043/
https://www.ncbi.nlm.nih.gov/pubmed/32933193
http://dx.doi.org/10.3390/ijms21186709
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author Bujak, Katarzyna
Decewicz, Przemyslaw
Kaminski, Jerzy
Radlinska, Monika
author_facet Bujak, Katarzyna
Decewicz, Przemyslaw
Kaminski, Jerzy
Radlinska, Monika
author_sort Bujak, Katarzyna
collection PubMed
description Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages.
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spelling pubmed-75560432020-10-19 Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine Bujak, Katarzyna Decewicz, Przemyslaw Kaminski, Jerzy Radlinska, Monika Int J Mol Sci Article Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages. MDPI 2020-09-13 /pmc/articles/PMC7556043/ /pubmed/32933193 http://dx.doi.org/10.3390/ijms21186709 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bujak, Katarzyna
Decewicz, Przemyslaw
Kaminski, Jerzy
Radlinska, Monika
Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title_full Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title_fullStr Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title_full_unstemmed Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title_short Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
title_sort identification, characterization, and genomic analysis of novel serratia temperate phages from a gold mine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556043/
https://www.ncbi.nlm.nih.gov/pubmed/32933193
http://dx.doi.org/10.3390/ijms21186709
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