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Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich envi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556043/ https://www.ncbi.nlm.nih.gov/pubmed/32933193 http://dx.doi.org/10.3390/ijms21186709 |
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author | Bujak, Katarzyna Decewicz, Przemyslaw Kaminski, Jerzy Radlinska, Monika |
author_facet | Bujak, Katarzyna Decewicz, Przemyslaw Kaminski, Jerzy Radlinska, Monika |
author_sort | Bujak, Katarzyna |
collection | PubMed |
description | Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages. |
format | Online Article Text |
id | pubmed-7556043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75560432020-10-19 Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine Bujak, Katarzyna Decewicz, Przemyslaw Kaminski, Jerzy Radlinska, Monika Int J Mol Sci Article Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages. MDPI 2020-09-13 /pmc/articles/PMC7556043/ /pubmed/32933193 http://dx.doi.org/10.3390/ijms21186709 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bujak, Katarzyna Decewicz, Przemyslaw Kaminski, Jerzy Radlinska, Monika Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title | Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title_full | Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title_fullStr | Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title_full_unstemmed | Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title_short | Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine |
title_sort | identification, characterization, and genomic analysis of novel serratia temperate phages from a gold mine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556043/ https://www.ncbi.nlm.nih.gov/pubmed/32933193 http://dx.doi.org/10.3390/ijms21186709 |
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