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17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway

Ketamine inhibits neural stem/progenitor cell (NSPC) proliferation and disrupts normal neurogenesis in the developing brain. 17β-Estradiol alleviates neurogenesis damage and enhances behavioral performance after ketamine administration. However, the receptor pathway of 17β-estradiol that protects NS...

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Autores principales: Li, Weisong, Lu, Pan, Lu, Yang, Wei, Haidong, Niu, Xiaoli, Xu, Jing, Wang, Kui, Zhang, Hong, Li, Rong, Qiu, Zhengguo, Wang, Ning, Jia, Pengyu, Zhang, Yan, Zhang, Shuyue, Lu, Haixia, Chen, Xinlin, Liu, Yong, Zhang, Pengbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556164/
https://www.ncbi.nlm.nih.gov/pubmed/33100963
http://dx.doi.org/10.3389/fnins.2020.576813
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author Li, Weisong
Lu, Pan
Lu, Yang
Wei, Haidong
Niu, Xiaoli
Xu, Jing
Wang, Kui
Zhang, Hong
Li, Rong
Qiu, Zhengguo
Wang, Ning
Jia, Pengyu
Zhang, Yan
Zhang, Shuyue
Lu, Haixia
Chen, Xinlin
Liu, Yong
Zhang, Pengbo
author_facet Li, Weisong
Lu, Pan
Lu, Yang
Wei, Haidong
Niu, Xiaoli
Xu, Jing
Wang, Kui
Zhang, Hong
Li, Rong
Qiu, Zhengguo
Wang, Ning
Jia, Pengyu
Zhang, Yan
Zhang, Shuyue
Lu, Haixia
Chen, Xinlin
Liu, Yong
Zhang, Pengbo
author_sort Li, Weisong
collection PubMed
description Ketamine inhibits neural stem/progenitor cell (NSPC) proliferation and disrupts normal neurogenesis in the developing brain. 17β-Estradiol alleviates neurogenesis damage and enhances behavioral performance after ketamine administration. However, the receptor pathway of 17β-estradiol that protects NSPCs from ketamine-induced injury remains unknown. In the present study, we investigated the role of estrogen receptor α (ER-α) and estrogen receptor β (ER-β) in 17β-estradiol’s protection against ketamine-exposed NSPCs and explored its potential mechanism. The primary cultured NSPCs were identified by immunofluorescence and then treated with ketamine and varying doses of ER-α agonist 4,4′,4″-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (PPT) or ER-β agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN) for 24 h. NSPC proliferation was analyzed by 5-bromo-2-deoxyuridine incorporation test. The expression of phosphorylated glycogen synthase kinase-3β (p-GSK-3β) was quantified by western blotting. It was found that treatment with different concentrations of PPT did not alter the inhibition of ketamine on NSPC proliferation. However, treatment with DPN attenuated the inhibition of ketamine on NSPC proliferation at 24 h after their exposure (P < 0.05). Furthermore, treatment with DPN increased p-GSK-3β expression in NSPCs exposed to ketamine. These findings indicated that ER-β mediates probably the protective effects of 17β-estradiol on ketamine-damaged NSPC proliferation and GSK-3β is involved in this process
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spelling pubmed-75561642020-10-22 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway Li, Weisong Lu, Pan Lu, Yang Wei, Haidong Niu, Xiaoli Xu, Jing Wang, Kui Zhang, Hong Li, Rong Qiu, Zhengguo Wang, Ning Jia, Pengyu Zhang, Yan Zhang, Shuyue Lu, Haixia Chen, Xinlin Liu, Yong Zhang, Pengbo Front Neurosci Neuroscience Ketamine inhibits neural stem/progenitor cell (NSPC) proliferation and disrupts normal neurogenesis in the developing brain. 17β-Estradiol alleviates neurogenesis damage and enhances behavioral performance after ketamine administration. However, the receptor pathway of 17β-estradiol that protects NSPCs from ketamine-induced injury remains unknown. In the present study, we investigated the role of estrogen receptor α (ER-α) and estrogen receptor β (ER-β) in 17β-estradiol’s protection against ketamine-exposed NSPCs and explored its potential mechanism. The primary cultured NSPCs were identified by immunofluorescence and then treated with ketamine and varying doses of ER-α agonist 4,4′,4″-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (PPT) or ER-β agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN) for 24 h. NSPC proliferation was analyzed by 5-bromo-2-deoxyuridine incorporation test. The expression of phosphorylated glycogen synthase kinase-3β (p-GSK-3β) was quantified by western blotting. It was found that treatment with different concentrations of PPT did not alter the inhibition of ketamine on NSPC proliferation. However, treatment with DPN attenuated the inhibition of ketamine on NSPC proliferation at 24 h after their exposure (P < 0.05). Furthermore, treatment with DPN increased p-GSK-3β expression in NSPCs exposed to ketamine. These findings indicated that ER-β mediates probably the protective effects of 17β-estradiol on ketamine-damaged NSPC proliferation and GSK-3β is involved in this process Frontiers Media S.A. 2020-09-30 /pmc/articles/PMC7556164/ /pubmed/33100963 http://dx.doi.org/10.3389/fnins.2020.576813 Text en Copyright © 2020 Li, Lu, Lu, Wei, Niu, Xu, Wang, Zhang, Li, Qiu, Wang, Jia, Zhang, Zhang, Lu, Chen, Liu and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Li, Weisong
Lu, Pan
Lu, Yang
Wei, Haidong
Niu, Xiaoli
Xu, Jing
Wang, Kui
Zhang, Hong
Li, Rong
Qiu, Zhengguo
Wang, Ning
Jia, Pengyu
Zhang, Yan
Zhang, Shuyue
Lu, Haixia
Chen, Xinlin
Liu, Yong
Zhang, Pengbo
17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title_full 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title_fullStr 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title_full_unstemmed 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title_short 17β-Estradiol Protects Neural Stem/Progenitor Cells Against Ketamine-Induced Injury Through Estrogen Receptor β Pathway
title_sort 17β-estradiol protects neural stem/progenitor cells against ketamine-induced injury through estrogen receptor β pathway
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556164/
https://www.ncbi.nlm.nih.gov/pubmed/33100963
http://dx.doi.org/10.3389/fnins.2020.576813
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