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Immunological pathways of macrophage response to Brucella ovis infection
As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis. The DEGs related to immune pathway were identified by Kyoto Encyclopedia o...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556187/ https://www.ncbi.nlm.nih.gov/pubmed/32970502 http://dx.doi.org/10.1177/1753425920958179 |
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author | Zhou, Zhixiong Gu, Guojing Luo, Yichen Li, Wenjie Li, Bowen Zhao, Yu Liu, Juan Shuai, Xuehong Wu, Li Chen, Jixuan Fan, Cailiang Huang, Qingzhou Han, Baoru Wen, Jianjun Jiao, Hanwei |
author_facet | Zhou, Zhixiong Gu, Guojing Luo, Yichen Li, Wenjie Li, Bowen Zhao, Yu Liu, Juan Shuai, Xuehong Wu, Li Chen, Jixuan Fan, Cailiang Huang, Qingzhou Han, Baoru Wen, Jianjun Jiao, Hanwei |
author_sort | Zhou, Zhixiong |
collection | PubMed |
description | As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis. The DEGs related to immune pathway were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) functional enrichment analysis. Quantitative real-time PCR (qRT-PCR) was performed to validate the transcriptome sequencing data. In total, we identified 337 up-regulated and 264 down-regulated DEGs in B. ovis-infected group versus mock group. Top 20 pathways were enriched by KEGG analysis and 20 GO by functional enrichment analysis in DEGs involved in the molecular function, cellular component, and biological process and so on, which revealed multiple immunological pathways in RAW264.7 macrophage cells in response to B. ovis infection, including inflammatory response, immune system process, immune response, cytokine activity, chemotaxis, chemokine-mediated signaling pathway, chemokine activity, and CCR chemokine receptor binding. qRT-PCR results showed Ccl2 (ENSMUST00000000193), Ccl2 (ENSMUST00000124479), Ccl3 (ENSMUST00000001008), Hmox1 (ENSMUST00000005548), Hmox1 (ENSMUST00000159631), Cxcl2 (ENSMUST00000075433), Cxcl2 (ENSMUST00000200681), Cxcl2 (ENSMUST00000200919), and Cxcl2 (ENSMUST00000202317). Our findings firstly elucidate the pathways involved in B. ovis-induced host immune response, which may lay the foundation for revealing the bacteria–host interaction and demonstrating the pathogenic mechanism of B. ovis. |
format | Online Article Text |
id | pubmed-7556187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-75561872020-10-26 Immunological pathways of macrophage response to Brucella ovis infection Zhou, Zhixiong Gu, Guojing Luo, Yichen Li, Wenjie Li, Bowen Zhao, Yu Liu, Juan Shuai, Xuehong Wu, Li Chen, Jixuan Fan, Cailiang Huang, Qingzhou Han, Baoru Wen, Jianjun Jiao, Hanwei Innate Immun Original Articles As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis. The DEGs related to immune pathway were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) functional enrichment analysis. Quantitative real-time PCR (qRT-PCR) was performed to validate the transcriptome sequencing data. In total, we identified 337 up-regulated and 264 down-regulated DEGs in B. ovis-infected group versus mock group. Top 20 pathways were enriched by KEGG analysis and 20 GO by functional enrichment analysis in DEGs involved in the molecular function, cellular component, and biological process and so on, which revealed multiple immunological pathways in RAW264.7 macrophage cells in response to B. ovis infection, including inflammatory response, immune system process, immune response, cytokine activity, chemotaxis, chemokine-mediated signaling pathway, chemokine activity, and CCR chemokine receptor binding. qRT-PCR results showed Ccl2 (ENSMUST00000000193), Ccl2 (ENSMUST00000124479), Ccl3 (ENSMUST00000001008), Hmox1 (ENSMUST00000005548), Hmox1 (ENSMUST00000159631), Cxcl2 (ENSMUST00000075433), Cxcl2 (ENSMUST00000200681), Cxcl2 (ENSMUST00000200919), and Cxcl2 (ENSMUST00000202317). Our findings firstly elucidate the pathways involved in B. ovis-induced host immune response, which may lay the foundation for revealing the bacteria–host interaction and demonstrating the pathogenic mechanism of B. ovis. SAGE Publications 2020-09-24 2020-10 /pmc/articles/PMC7556187/ /pubmed/32970502 http://dx.doi.org/10.1177/1753425920958179 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Zhou, Zhixiong Gu, Guojing Luo, Yichen Li, Wenjie Li, Bowen Zhao, Yu Liu, Juan Shuai, Xuehong Wu, Li Chen, Jixuan Fan, Cailiang Huang, Qingzhou Han, Baoru Wen, Jianjun Jiao, Hanwei Immunological pathways of macrophage response to Brucella ovis infection |
title | Immunological pathways of macrophage response to Brucella ovis infection |
title_full | Immunological pathways of macrophage response to Brucella ovis infection |
title_fullStr | Immunological pathways of macrophage response to Brucella ovis infection |
title_full_unstemmed | Immunological pathways of macrophage response to Brucella ovis infection |
title_short | Immunological pathways of macrophage response to Brucella ovis infection |
title_sort | immunological pathways of macrophage response to brucella ovis infection |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556187/ https://www.ncbi.nlm.nih.gov/pubmed/32970502 http://dx.doi.org/10.1177/1753425920958179 |
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