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Progress and applications of mass cytometry in sketching immune landscapes
Recently emerged mass cytometry (cytometry by time‐of‐flight [CyTOF]) technology permits the identification and quantification of inherently diverse cellular systems, and the simultaneous measurement of functional attributes at the single‐cell resolution. By virtue of its multiplex ability with limi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556381/ https://www.ncbi.nlm.nih.gov/pubmed/33135337 http://dx.doi.org/10.1002/ctm2.206 |
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author | Zhang, Ting Warden, Antony R. Li, Yiyang Ding, Xianting |
author_facet | Zhang, Ting Warden, Antony R. Li, Yiyang Ding, Xianting |
author_sort | Zhang, Ting |
collection | PubMed |
description | Recently emerged mass cytometry (cytometry by time‐of‐flight [CyTOF]) technology permits the identification and quantification of inherently diverse cellular systems, and the simultaneous measurement of functional attributes at the single‐cell resolution. By virtue of its multiplex ability with limited need for compensation, CyTOF has led a critical role in immunological research fields. Here, we present an overview of CyTOF, including the introduction of CyTOF principle and advantages that make it a standalone tool in deciphering immune mysteries. We then discuss the functional assays, introduce the bioinformatics to interpret the data yield via CyTOF, and depict the emerging clinical and research applications of CyTOF technology in sketching immune landscape in a wide variety of diseases. |
format | Online Article Text |
id | pubmed-7556381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75563812020-10-19 Progress and applications of mass cytometry in sketching immune landscapes Zhang, Ting Warden, Antony R. Li, Yiyang Ding, Xianting Clin Transl Med Reviews Recently emerged mass cytometry (cytometry by time‐of‐flight [CyTOF]) technology permits the identification and quantification of inherently diverse cellular systems, and the simultaneous measurement of functional attributes at the single‐cell resolution. By virtue of its multiplex ability with limited need for compensation, CyTOF has led a critical role in immunological research fields. Here, we present an overview of CyTOF, including the introduction of CyTOF principle and advantages that make it a standalone tool in deciphering immune mysteries. We then discuss the functional assays, introduce the bioinformatics to interpret the data yield via CyTOF, and depict the emerging clinical and research applications of CyTOF technology in sketching immune landscape in a wide variety of diseases. John Wiley and Sons Inc. 2020-10-14 /pmc/articles/PMC7556381/ /pubmed/33135337 http://dx.doi.org/10.1002/ctm2.206 Text en © 2020 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reviews Zhang, Ting Warden, Antony R. Li, Yiyang Ding, Xianting Progress and applications of mass cytometry in sketching immune landscapes |
title | Progress and applications of mass cytometry in sketching immune landscapes |
title_full | Progress and applications of mass cytometry in sketching immune landscapes |
title_fullStr | Progress and applications of mass cytometry in sketching immune landscapes |
title_full_unstemmed | Progress and applications of mass cytometry in sketching immune landscapes |
title_short | Progress and applications of mass cytometry in sketching immune landscapes |
title_sort | progress and applications of mass cytometry in sketching immune landscapes |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556381/ https://www.ncbi.nlm.nih.gov/pubmed/33135337 http://dx.doi.org/10.1002/ctm2.206 |
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