Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step

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The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic testing. The World Health Organization (WHO) recommends a standard assay that includes an RNA extraction step from a nasopharyngeal (NP) swab followed by reverse transcription–quantitative polymerase chain reaction...

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Detalles Bibliográficos
Autores principales: Bruce, Emily A., Huang, Meei-Li, Perchetti, Garrett A., Tighe, Scott, Laaguiby, Pheobe, Hoffman, Jessica J., Gerrard, Diana L., Nalla, Arun K., Wei, Yulun, Greninger, Alexander L., Diehl, Sean A., Shirley, David J., Leonard, Debra G. B., Huston, Christopher D., Kirkpatrick, Beth D., Dragon, Julie A., Crothers, Jessica W., Jerome, Keith R., Botten, Jason W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556528/
https://www.ncbi.nlm.nih.gov/pubmed/33006983
http://dx.doi.org/10.1371/journal.pbio.3000896
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