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Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis

The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside...

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Autores principales: Campolina, Thais Bonifácio, Villegas, Luis Eduardo Martinez, Monteiro, Carolina Cunha, Pimenta, Paulo Filemon Paolucci, Secundino, Nagila Francinete Costa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556539/
https://www.ncbi.nlm.nih.gov/pubmed/33052941
http://dx.doi.org/10.1371/journal.pntd.0008666
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author Campolina, Thais Bonifácio
Villegas, Luis Eduardo Martinez
Monteiro, Carolina Cunha
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author_facet Campolina, Thais Bonifácio
Villegas, Luis Eduardo Martinez
Monteiro, Carolina Cunha
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author_sort Campolina, Thais Bonifácio
collection PubMed
description The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania.
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spelling pubmed-75565392020-10-21 Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis Campolina, Thais Bonifácio Villegas, Luis Eduardo Martinez Monteiro, Carolina Cunha Pimenta, Paulo Filemon Paolucci Secundino, Nagila Francinete Costa PLoS Negl Trop Dis Research Article The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania. Public Library of Science 2020-10-14 /pmc/articles/PMC7556539/ /pubmed/33052941 http://dx.doi.org/10.1371/journal.pntd.0008666 Text en © 2020 Campolina et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Campolina, Thais Bonifácio
Villegas, Luis Eduardo Martinez
Monteiro, Carolina Cunha
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title_full Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title_fullStr Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title_full_unstemmed Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title_short Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis
title_sort tripartite interactions: leishmania, microbiota and lutzomyia longipalpis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556539/
https://www.ncbi.nlm.nih.gov/pubmed/33052941
http://dx.doi.org/10.1371/journal.pntd.0008666
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