Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA u...

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Detalles Bibliográficos
Autores principales: Behrmann, Ole, Bachmann, Iris, Hufert, Frank, Dame, Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Wissenschaft Methoden
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556600/
https://www.ncbi.nlm.nih.gov/pubmed/33078045
http://dx.doi.org/10.1007/s12268-020-1458-3
Descripción
Sumario:The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

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