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Methods for extracellular vesicle isolation from cancer cells
Cells are known to release different types of vesicles such as small extracellular vesicles (sEVs) and large extracellular vesicles (LEVs). sEVs and LEVs play important roles in intercellular communication, pre-metastatic niche formation, and disease progression; both can be detected cell culture me...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
OAE Publishing Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556721/ https://www.ncbi.nlm.nih.gov/pubmed/33062957 http://dx.doi.org/10.20517/cdr.2019.118 |
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author | Salem, Israa Naranjo, Nicole M. Singh, Amrita DeRita, Rachel Krishn, Shiv Ram Sirman, Luca S. Quaglia, Fabio Duffy, Alexander Bowler, Nicholas Sayeed, Aejaz Languino, Lucia R. |
author_facet | Salem, Israa Naranjo, Nicole M. Singh, Amrita DeRita, Rachel Krishn, Shiv Ram Sirman, Luca S. Quaglia, Fabio Duffy, Alexander Bowler, Nicholas Sayeed, Aejaz Languino, Lucia R. |
author_sort | Salem, Israa |
collection | PubMed |
description | Cells are known to release different types of vesicles such as small extracellular vesicles (sEVs) and large extracellular vesicles (LEVs). sEVs and LEVs play important roles in intercellular communication, pre-metastatic niche formation, and disease progression; both can be detected cell culture media and biological fluids. sEVs and LEVs contain a variety of protein and RNA cargo, and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface proteins. It has recently been established that standard isolation techniques, such as differential ultracentrifugation, yield a mixed population of EVs. However, density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular debris. Here, we describe the most common methods used to isolate sEVs from cell culture medium, mouse and human plasma, and a new technique for isolating sEVs from tissues as well. This article also provides detailed procedures to isolate LEVs. |
format | Online Article Text |
id | pubmed-7556721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | OAE Publishing Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75567212020-10-14 Methods for extracellular vesicle isolation from cancer cells Salem, Israa Naranjo, Nicole M. Singh, Amrita DeRita, Rachel Krishn, Shiv Ram Sirman, Luca S. Quaglia, Fabio Duffy, Alexander Bowler, Nicholas Sayeed, Aejaz Languino, Lucia R. Cancer Drug Resist Review Cells are known to release different types of vesicles such as small extracellular vesicles (sEVs) and large extracellular vesicles (LEVs). sEVs and LEVs play important roles in intercellular communication, pre-metastatic niche formation, and disease progression; both can be detected cell culture media and biological fluids. sEVs and LEVs contain a variety of protein and RNA cargo, and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface proteins. It has recently been established that standard isolation techniques, such as differential ultracentrifugation, yield a mixed population of EVs. However, density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular debris. Here, we describe the most common methods used to isolate sEVs from cell culture medium, mouse and human plasma, and a new technique for isolating sEVs from tissues as well. This article also provides detailed procedures to isolate LEVs. OAE Publishing Inc. 2020-04-25 /pmc/articles/PMC7556721/ /pubmed/33062957 http://dx.doi.org/10.20517/cdr.2019.118 Text en © The Author(s) 2020. https://creativecommons.org/licenses/by/4.0/© The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Review Salem, Israa Naranjo, Nicole M. Singh, Amrita DeRita, Rachel Krishn, Shiv Ram Sirman, Luca S. Quaglia, Fabio Duffy, Alexander Bowler, Nicholas Sayeed, Aejaz Languino, Lucia R. Methods for extracellular vesicle isolation from cancer cells |
title | Methods for extracellular vesicle isolation from cancer cells |
title_full | Methods for extracellular vesicle isolation from cancer cells |
title_fullStr | Methods for extracellular vesicle isolation from cancer cells |
title_full_unstemmed | Methods for extracellular vesicle isolation from cancer cells |
title_short | Methods for extracellular vesicle isolation from cancer cells |
title_sort | methods for extracellular vesicle isolation from cancer cells |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556721/ https://www.ncbi.nlm.nih.gov/pubmed/33062957 http://dx.doi.org/10.20517/cdr.2019.118 |
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