Cargando…
The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo
Cell migration is a dynamic process that entails extensive protein synthesis and recycling, structural remodeling, and considerable bioenergetic demand. Autophagy is one of the pathways that maintain cellular homeostasis. Time-lapse imaging of autophagosomes and ATP/ADP levels in migrating cells in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556871/ https://www.ncbi.nlm.nih.gov/pubmed/32985978 http://dx.doi.org/10.7554/eLife.56006 |
_version_ | 1783594301973856256 |
---|---|
author | Bressan, Cedric Pecora, Alessandra Gagnon, Dave Snapyan, Marina Labrecque, Simon De Koninck, Paul Parent, Martin Saghatelyan, Armen |
author_facet | Bressan, Cedric Pecora, Alessandra Gagnon, Dave Snapyan, Marina Labrecque, Simon De Koninck, Paul Parent, Martin Saghatelyan, Armen |
author_sort | Bressan, Cedric |
collection | PubMed |
description | Cell migration is a dynamic process that entails extensive protein synthesis and recycling, structural remodeling, and considerable bioenergetic demand. Autophagy is one of the pathways that maintain cellular homeostasis. Time-lapse imaging of autophagosomes and ATP/ADP levels in migrating cells in the rostral migratory stream of mouse revealed that decreases in ATP levels force cells into the stationary phase and induce autophagy. Pharmacological or genetic impairments of autophagy in neuroblasts using either bafilomycin, inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer duration of the stationary phase. Autophagy is modulated in response to migration-promoting and inhibiting molecular cues and is required for the recycling of focal adhesions. Our results show that autophagy and energy consumption act in concert in migrating cells to dynamically regulate the pace and periodicity of the migratory and stationary phases to sustain neuronal migration. |
format | Online Article Text |
id | pubmed-7556871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75568712020-10-16 The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo Bressan, Cedric Pecora, Alessandra Gagnon, Dave Snapyan, Marina Labrecque, Simon De Koninck, Paul Parent, Martin Saghatelyan, Armen eLife Neuroscience Cell migration is a dynamic process that entails extensive protein synthesis and recycling, structural remodeling, and considerable bioenergetic demand. Autophagy is one of the pathways that maintain cellular homeostasis. Time-lapse imaging of autophagosomes and ATP/ADP levels in migrating cells in the rostral migratory stream of mouse revealed that decreases in ATP levels force cells into the stationary phase and induce autophagy. Pharmacological or genetic impairments of autophagy in neuroblasts using either bafilomycin, inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer duration of the stationary phase. Autophagy is modulated in response to migration-promoting and inhibiting molecular cues and is required for the recycling of focal adhesions. Our results show that autophagy and energy consumption act in concert in migrating cells to dynamically regulate the pace and periodicity of the migratory and stationary phases to sustain neuronal migration. eLife Sciences Publications, Ltd 2020-09-28 /pmc/articles/PMC7556871/ /pubmed/32985978 http://dx.doi.org/10.7554/eLife.56006 Text en © 2020, Bressan et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Bressan, Cedric Pecora, Alessandra Gagnon, Dave Snapyan, Marina Labrecque, Simon De Koninck, Paul Parent, Martin Saghatelyan, Armen The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title | The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title_full | The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title_fullStr | The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title_full_unstemmed | The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title_short | The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo |
title_sort | dynamic interplay between atp/adp levels and autophagy sustain neuronal migration in vivo |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556871/ https://www.ncbi.nlm.nih.gov/pubmed/32985978 http://dx.doi.org/10.7554/eLife.56006 |
work_keys_str_mv | AT bressancedric thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT pecoraalessandra thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT gagnondave thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT snapyanmarina thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT labrecquesimon thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT dekoninckpaul thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT parentmartin thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT saghatelyanarmen thedynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT bressancedric dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT pecoraalessandra dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT gagnondave dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT snapyanmarina dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT labrecquesimon dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT dekoninckpaul dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT parentmartin dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo AT saghatelyanarmen dynamicinterplaybetweenatpadplevelsandautophagysustainneuronalmigrationinvivo |