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Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome

Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation...

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Autores principales: Ali, Md. Hossen, Sobze, Jean-Marie, Pham, Thu Huong, Nadeem, Muhammad, Liu, Chen, Galagedara, Lakshman, Cheema, Mumtaz, Thomas, Raymond
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557389/
https://www.ncbi.nlm.nih.gov/pubmed/32947854
http://dx.doi.org/10.3390/nano10091852
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author Ali, Md. Hossen
Sobze, Jean-Marie
Pham, Thu Huong
Nadeem, Muhammad
Liu, Chen
Galagedara, Lakshman
Cheema, Mumtaz
Thomas, Raymond
author_facet Ali, Md. Hossen
Sobze, Jean-Marie
Pham, Thu Huong
Nadeem, Muhammad
Liu, Chen
Galagedara, Lakshman
Cheema, Mumtaz
Thomas, Raymond
author_sort Ali, Md. Hossen
collection PubMed
description Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation or vigor for forest reclamation activities. Herein, we demonstrate the use of nanopriming with carbon nanotubes (CNT) to alleviate seed dormancy, improved seed germination, and seedling vigor in two boreal peatland species. Bog birch (Betula pumila L.) and Labrador tea (Rhododendron groenlandicum L.) seeds with embryo or seed coat dormancy were nanoprimed with either 20 or 40 µg/mL CNT, cold stratified at 2–4 °C for 15 days, and allowed to germinate at room temperature. The emerged seedlings’ lipidome was assessed to decipher the role of lipid metabolism in alleviating seed dormancy. We observed significant (p < 0.05) improvement in seedling germination and seedling vigor in seeds primed with multiwalled carbon nanotubes functionalized with carboxylic acids. Phosphatidylcholine (PC 18:1/18:3), phosphatidylglycerol (PG 16:1/18:3), and lysophosphatidylcholine (LPC 18:3) molecular species (C18:3 enriched) were observed to be highly correlated with the increased seed germination percentages and the enhanced seedling vigor. Mechanistically, it appears that carbon nanoprimed seeds following stratification are effective in mediating seed dormancy by remodeling the seed membrane lipids (C18:3 enriched PC, PG, and LPC) in both peatland boreal forest species. The study results demonstrate that nanopriming may provide a solution to resolve seed dormancy issues by enhancing seed germination, propagation, and seedling vigor in non-resource boreal forest species ideally suited for forest reclamation following anthropogenic disturbances.
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spelling pubmed-75573892020-10-20 Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome Ali, Md. Hossen Sobze, Jean-Marie Pham, Thu Huong Nadeem, Muhammad Liu, Chen Galagedara, Lakshman Cheema, Mumtaz Thomas, Raymond Nanomaterials (Basel) Article Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation or vigor for forest reclamation activities. Herein, we demonstrate the use of nanopriming with carbon nanotubes (CNT) to alleviate seed dormancy, improved seed germination, and seedling vigor in two boreal peatland species. Bog birch (Betula pumila L.) and Labrador tea (Rhododendron groenlandicum L.) seeds with embryo or seed coat dormancy were nanoprimed with either 20 or 40 µg/mL CNT, cold stratified at 2–4 °C for 15 days, and allowed to germinate at room temperature. The emerged seedlings’ lipidome was assessed to decipher the role of lipid metabolism in alleviating seed dormancy. We observed significant (p < 0.05) improvement in seedling germination and seedling vigor in seeds primed with multiwalled carbon nanotubes functionalized with carboxylic acids. Phosphatidylcholine (PC 18:1/18:3), phosphatidylglycerol (PG 16:1/18:3), and lysophosphatidylcholine (LPC 18:3) molecular species (C18:3 enriched) were observed to be highly correlated with the increased seed germination percentages and the enhanced seedling vigor. Mechanistically, it appears that carbon nanoprimed seeds following stratification are effective in mediating seed dormancy by remodeling the seed membrane lipids (C18:3 enriched PC, PG, and LPC) in both peatland boreal forest species. The study results demonstrate that nanopriming may provide a solution to resolve seed dormancy issues by enhancing seed germination, propagation, and seedling vigor in non-resource boreal forest species ideally suited for forest reclamation following anthropogenic disturbances. MDPI 2020-09-16 /pmc/articles/PMC7557389/ /pubmed/32947854 http://dx.doi.org/10.3390/nano10091852 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ali, Md. Hossen
Sobze, Jean-Marie
Pham, Thu Huong
Nadeem, Muhammad
Liu, Chen
Galagedara, Lakshman
Cheema, Mumtaz
Thomas, Raymond
Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title_full Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title_fullStr Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title_full_unstemmed Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title_short Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
title_sort carbon nanotubes improved the germination and vigor of plant species from peatland ecosystem via remodeling the membrane lipidome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557389/
https://www.ncbi.nlm.nih.gov/pubmed/32947854
http://dx.doi.org/10.3390/nano10091852
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