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Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and ei...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557755/ https://www.ncbi.nlm.nih.gov/pubmed/32872452 http://dx.doi.org/10.3390/vetsci7030121 |
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author | Šefcová, Miroslava Larrea-Álvarez, Marco Larrea-Álvarez, César Revajová, Viera Karaffová, Viera Koščová, Jana Nemcová, Radomíra Ortega-Paredes, David Vinueza-Burgos, Christian Levkut, Mikuláš Herich, Róbert |
author_facet | Šefcová, Miroslava Larrea-Álvarez, Marco Larrea-Álvarez, César Revajová, Viera Karaffová, Viera Koščová, Jana Nemcová, Radomíra Ortega-Paredes, David Vinueza-Burgos, Christian Levkut, Mikuláš Herich, Róbert |
author_sort | Šefcová, Miroslava |
collection | PubMed |
description | Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production. |
format | Online Article Text |
id | pubmed-7557755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75577552020-10-20 Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens Šefcová, Miroslava Larrea-Álvarez, Marco Larrea-Álvarez, César Revajová, Viera Karaffová, Viera Koščová, Jana Nemcová, Radomíra Ortega-Paredes, David Vinueza-Burgos, Christian Levkut, Mikuláš Herich, Róbert Vet Sci Communication Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production. MDPI 2020-08-29 /pmc/articles/PMC7557755/ /pubmed/32872452 http://dx.doi.org/10.3390/vetsci7030121 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Šefcová, Miroslava Larrea-Álvarez, Marco Larrea-Álvarez, César Revajová, Viera Karaffová, Viera Koščová, Jana Nemcová, Radomíra Ortega-Paredes, David Vinueza-Burgos, Christian Levkut, Mikuláš Herich, Róbert Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title | Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title_full | Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title_fullStr | Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title_full_unstemmed | Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title_short | Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens |
title_sort | effects of lactobacillus fermentum supplementation on body weight and pro-inflammatory cytokine expression in campylobacter jejuni-challenged chickens |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557755/ https://www.ncbi.nlm.nih.gov/pubmed/32872452 http://dx.doi.org/10.3390/vetsci7030121 |
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