Cargando…

Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens

Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and ei...

Descripción completa

Detalles Bibliográficos
Autores principales: Šefcová, Miroslava, Larrea-Álvarez, Marco, Larrea-Álvarez, César, Revajová, Viera, Karaffová, Viera, Koščová, Jana, Nemcová, Radomíra, Ortega-Paredes, David, Vinueza-Burgos, Christian, Levkut, Mikuláš, Herich, Róbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557755/
https://www.ncbi.nlm.nih.gov/pubmed/32872452
http://dx.doi.org/10.3390/vetsci7030121
_version_ 1783594486074441728
author Šefcová, Miroslava
Larrea-Álvarez, Marco
Larrea-Álvarez, César
Revajová, Viera
Karaffová, Viera
Koščová, Jana
Nemcová, Radomíra
Ortega-Paredes, David
Vinueza-Burgos, Christian
Levkut, Mikuláš
Herich, Róbert
author_facet Šefcová, Miroslava
Larrea-Álvarez, Marco
Larrea-Álvarez, César
Revajová, Viera
Karaffová, Viera
Koščová, Jana
Nemcová, Radomíra
Ortega-Paredes, David
Vinueza-Burgos, Christian
Levkut, Mikuláš
Herich, Róbert
author_sort Šefcová, Miroslava
collection PubMed
description Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production.
format Online
Article
Text
id pubmed-7557755
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75577552020-10-20 Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens Šefcová, Miroslava Larrea-Álvarez, Marco Larrea-Álvarez, César Revajová, Viera Karaffová, Viera Koščová, Jana Nemcová, Radomíra Ortega-Paredes, David Vinueza-Burgos, Christian Levkut, Mikuláš Herich, Róbert Vet Sci Communication Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production. MDPI 2020-08-29 /pmc/articles/PMC7557755/ /pubmed/32872452 http://dx.doi.org/10.3390/vetsci7030121 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Šefcová, Miroslava
Larrea-Álvarez, Marco
Larrea-Álvarez, César
Revajová, Viera
Karaffová, Viera
Koščová, Jana
Nemcová, Radomíra
Ortega-Paredes, David
Vinueza-Burgos, Christian
Levkut, Mikuláš
Herich, Róbert
Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title_full Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title_fullStr Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title_full_unstemmed Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title_short Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens
title_sort effects of lactobacillus fermentum supplementation on body weight and pro-inflammatory cytokine expression in campylobacter jejuni-challenged chickens
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557755/
https://www.ncbi.nlm.nih.gov/pubmed/32872452
http://dx.doi.org/10.3390/vetsci7030121
work_keys_str_mv AT sefcovamiroslava effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT larreaalvarezmarco effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT larreaalvarezcesar effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT revajovaviera effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT karaffovaviera effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT koscovajana effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT nemcovaradomira effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT ortegaparedesdavid effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT vinuezaburgoschristian effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT levkutmikulas effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens
AT herichrobert effectsoflactobacillusfermentumsupplementationonbodyweightandproinflammatorycytokineexpressionincampylobacterjejunichallengedchickens