Cargando…
Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles
A green and cost-effective technique for the preparation of silver nanoparticles (Algae-AgNPs) as a colorimetric sensor for hydrogen peroxide (H(2)O(2)) is described. Silver nanoparticles were capped using the green algae (Noctiluca scintillans) extract at an optimum time of 3 h at 80 °C. The pH of...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557820/ https://www.ncbi.nlm.nih.gov/pubmed/32957557 http://dx.doi.org/10.3390/nano10091861 |
_version_ | 1783594501403574272 |
---|---|
author | Elgamouz, Abdelaziz Idriss, Hamid Nassab, Chahlaa Bihi, Alaa Bajou, Khalid Hasan, Kamrul Abu Haija, Mohammad Patole, Shashikant P. |
author_facet | Elgamouz, Abdelaziz Idriss, Hamid Nassab, Chahlaa Bihi, Alaa Bajou, Khalid Hasan, Kamrul Abu Haija, Mohammad Patole, Shashikant P. |
author_sort | Elgamouz, Abdelaziz |
collection | PubMed |
description | A green and cost-effective technique for the preparation of silver nanoparticles (Algae-AgNPs) as a colorimetric sensor for hydrogen peroxide (H(2)O(2)) is described. Silver nanoparticles were capped using the green algae (Noctiluca scintillans) extract at an optimum time of 3 h at 80 °C. The pH of the plant extract (pH = 7.0) yields nanoparticles with a mean size of 4.13 nm and a zeta potential of 0.200 ± 0.02 mV and negative polarity, using dynamic light scattering (DLS). High-resolution transmission electron microscopy (HRTEM) analysis showed regular spherical particles with the average size of 4.5 nm. Selected area electron diffraction (SAED) results revealed the polycrystalline nature of the silver nanoparticles. The obtained patterns were indexed as (111), (200), (220), and (311) reflections of the fcc (face centered cubic) silver crystal based on their d-spacing of 2.47, 2.13, 1.49, and 1.27 Å, respectively. The apparent color change from brown to colorless was observed when nanoparticles reacted with H(2)O(2). Linear responses were obtained in three different ranges (nM, µM, and mM). Limits of detection (LOD) of 1.33 ± 0.02 and 1.77 ± 0.02 nM and quantitation limits (LOQ) of 7.31 ± 0.03 and 9.67 ± 0.03 nM were obtained for Abs and ΔAbs calibration curves, respectively. 10% v/v Algae-AgNPs solution inhibited Staphylococcus aureus over Escherichia coli, while a 50% reduction of tumor cell growth of MDA-MB-231 human breast adenocarcinoma was obtained. |
format | Online Article Text |
id | pubmed-7557820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75578202020-10-22 Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles Elgamouz, Abdelaziz Idriss, Hamid Nassab, Chahlaa Bihi, Alaa Bajou, Khalid Hasan, Kamrul Abu Haija, Mohammad Patole, Shashikant P. Nanomaterials (Basel) Article A green and cost-effective technique for the preparation of silver nanoparticles (Algae-AgNPs) as a colorimetric sensor for hydrogen peroxide (H(2)O(2)) is described. Silver nanoparticles were capped using the green algae (Noctiluca scintillans) extract at an optimum time of 3 h at 80 °C. The pH of the plant extract (pH = 7.0) yields nanoparticles with a mean size of 4.13 nm and a zeta potential of 0.200 ± 0.02 mV and negative polarity, using dynamic light scattering (DLS). High-resolution transmission electron microscopy (HRTEM) analysis showed regular spherical particles with the average size of 4.5 nm. Selected area electron diffraction (SAED) results revealed the polycrystalline nature of the silver nanoparticles. The obtained patterns were indexed as (111), (200), (220), and (311) reflections of the fcc (face centered cubic) silver crystal based on their d-spacing of 2.47, 2.13, 1.49, and 1.27 Å, respectively. The apparent color change from brown to colorless was observed when nanoparticles reacted with H(2)O(2). Linear responses were obtained in three different ranges (nM, µM, and mM). Limits of detection (LOD) of 1.33 ± 0.02 and 1.77 ± 0.02 nM and quantitation limits (LOQ) of 7.31 ± 0.03 and 9.67 ± 0.03 nM were obtained for Abs and ΔAbs calibration curves, respectively. 10% v/v Algae-AgNPs solution inhibited Staphylococcus aureus over Escherichia coli, while a 50% reduction of tumor cell growth of MDA-MB-231 human breast adenocarcinoma was obtained. MDPI 2020-09-17 /pmc/articles/PMC7557820/ /pubmed/32957557 http://dx.doi.org/10.3390/nano10091861 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Elgamouz, Abdelaziz Idriss, Hamid Nassab, Chahlaa Bihi, Alaa Bajou, Khalid Hasan, Kamrul Abu Haija, Mohammad Patole, Shashikant P. Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title | Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title_full | Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title_fullStr | Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title_full_unstemmed | Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title_short | Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles |
title_sort | green synthesis, characterization, antimicrobial, anti-cancer, and optimization of colorimetric sensing of hydrogen peroxide of algae extract capped silver nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557820/ https://www.ncbi.nlm.nih.gov/pubmed/32957557 http://dx.doi.org/10.3390/nano10091861 |
work_keys_str_mv | AT elgamouzabdelaziz greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT idrisshamid greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT nassabchahlaa greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT bihialaa greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT bajoukhalid greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT hasankamrul greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT abuhaijamohammad greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles AT patoleshashikantp greensynthesischaracterizationantimicrobialanticancerandoptimizationofcolorimetricsensingofhydrogenperoxideofalgaeextractcappedsilvernanoparticles |