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Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases
This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Tra...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557837/ https://www.ncbi.nlm.nih.gov/pubmed/32872140 http://dx.doi.org/10.3390/ph13090216 |
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author | Palmas, Lucia Aroffu, Matteo Petretto, Giacomo L. Escribano-Ferrer, Elvira Díez-Sales, Octavio Usach, Iris Peris, José-Esteban Marongiu, Francesca Ghavam, Mansureh Fais, Sara Orrù, Germano Rached, Rita Abi Manca, Maria Letizia Manconi, Maria |
author_facet | Palmas, Lucia Aroffu, Matteo Petretto, Giacomo L. Escribano-Ferrer, Elvira Díez-Sales, Octavio Usach, Iris Peris, José-Esteban Marongiu, Francesca Ghavam, Mansureh Fais, Sara Orrù, Germano Rached, Rita Abi Manca, Maria Letizia Manconi, Maria |
author_sort | Palmas, Lucia |
collection | PubMed |
description | This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Transparent vesicle dispersions were obtained by direct sonication avoiding the use of organic solvents. Cryogenic transmission electron microscopy (cryo-TEM) confirmed the formation of unilamellar, spherical and regularly shaped vesicles. Essential oil and citral loaded liposomes were small in size (~110 and ~100 nm, respectively) and negatively charged. Liposomes, especially those loading citral, were highly stable as their physico-chemical properties did not change during storage. The formulations were highly biocompatible against keratinocytes, were able to counteract the damages induced in cells by using hydrogen peroxide, and able to increase the rate of skin repair. In addition, liposomes loading citral at higher concentrations inhibited the proliferation of cariogenic bacterium. |
format | Online Article Text |
id | pubmed-7557837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75578372020-10-22 Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases Palmas, Lucia Aroffu, Matteo Petretto, Giacomo L. Escribano-Ferrer, Elvira Díez-Sales, Octavio Usach, Iris Peris, José-Esteban Marongiu, Francesca Ghavam, Mansureh Fais, Sara Orrù, Germano Rached, Rita Abi Manca, Maria Letizia Manconi, Maria Pharmaceuticals (Basel) Article This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Transparent vesicle dispersions were obtained by direct sonication avoiding the use of organic solvents. Cryogenic transmission electron microscopy (cryo-TEM) confirmed the formation of unilamellar, spherical and regularly shaped vesicles. Essential oil and citral loaded liposomes were small in size (~110 and ~100 nm, respectively) and negatively charged. Liposomes, especially those loading citral, were highly stable as their physico-chemical properties did not change during storage. The formulations were highly biocompatible against keratinocytes, were able to counteract the damages induced in cells by using hydrogen peroxide, and able to increase the rate of skin repair. In addition, liposomes loading citral at higher concentrations inhibited the proliferation of cariogenic bacterium. MDPI 2020-08-28 /pmc/articles/PMC7557837/ /pubmed/32872140 http://dx.doi.org/10.3390/ph13090216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Palmas, Lucia Aroffu, Matteo Petretto, Giacomo L. Escribano-Ferrer, Elvira Díez-Sales, Octavio Usach, Iris Peris, José-Esteban Marongiu, Francesca Ghavam, Mansureh Fais, Sara Orrù, Germano Rached, Rita Abi Manca, Maria Letizia Manconi, Maria Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title | Entrapment of Citrus
limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title_full | Entrapment of Citrus
limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title_fullStr | Entrapment of Citrus
limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title_full_unstemmed | Entrapment of Citrus
limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title_short | Entrapment of Citrus
limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases |
title_sort | entrapment of citrus
limon var. pompia essential oil or pure citral in liposomes tailored as mouthwash for the treatment of oral cavity diseases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557837/ https://www.ncbi.nlm.nih.gov/pubmed/32872140 http://dx.doi.org/10.3390/ph13090216 |
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