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Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy

Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological...

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Autores principales: Montalbano, Giorgia, Borciani, Giorgia, Cerqueni, Giorgia, Licini, Caterina, Banche-Niclot, Federica, Janner, Davide, Sola, Stefania, Fiorilli, Sonia, Mattioli-Belmonte, Monica, Ciapetti, Gabriela, Vitale-Brovarone, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558137/
https://www.ncbi.nlm.nih.gov/pubmed/32867075
http://dx.doi.org/10.3390/nano10091681
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author Montalbano, Giorgia
Borciani, Giorgia
Cerqueni, Giorgia
Licini, Caterina
Banche-Niclot, Federica
Janner, Davide
Sola, Stefania
Fiorilli, Sonia
Mattioli-Belmonte, Monica
Ciapetti, Gabriela
Vitale-Brovarone, Chiara
author_facet Montalbano, Giorgia
Borciani, Giorgia
Cerqueni, Giorgia
Licini, Caterina
Banche-Niclot, Federica
Janner, Davide
Sola, Stefania
Fiorilli, Sonia
Mattioli-Belmonte, Monica
Ciapetti, Gabriela
Vitale-Brovarone, Chiara
author_sort Montalbano, Giorgia
collection PubMed
description Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological environment: By mimicking the natural features of bone tissue, the cell behavior during the regeneration process may be addressed. At present, 3D-printing technologies are the only techniques able to design complex structures avoiding constraints of final shape and porosity. However, this type of biofabrication requires complex optimization of biomaterial formulations in terms of specific rheological and mechanical properties while preserving high biocompatibility. In this work, we combined nano-sized mesoporous bioactive glasses enriched with strontium ions with type I collagen, to formulate a bioactive ink for 3D-printing technologies. Moreover, to avoid the premature release of strontium ions within the crosslinking medium and to significantly increase the material mechanical and thermal stability, we applied an optimized chemical treatment using ethanol-dissolved genipin solutions. The high biocompatibility of the hybrid system was confirmed by using MG-63 and Saos-2 osteoblast-like cell lines, further highlighting the great potential of the innovative nanocomposite for the design of bone-like scaffolds.
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spelling pubmed-75581372020-10-29 Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy Montalbano, Giorgia Borciani, Giorgia Cerqueni, Giorgia Licini, Caterina Banche-Niclot, Federica Janner, Davide Sola, Stefania Fiorilli, Sonia Mattioli-Belmonte, Monica Ciapetti, Gabriela Vitale-Brovarone, Chiara Nanomaterials (Basel) Article Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological environment: By mimicking the natural features of bone tissue, the cell behavior during the regeneration process may be addressed. At present, 3D-printing technologies are the only techniques able to design complex structures avoiding constraints of final shape and porosity. However, this type of biofabrication requires complex optimization of biomaterial formulations in terms of specific rheological and mechanical properties while preserving high biocompatibility. In this work, we combined nano-sized mesoporous bioactive glasses enriched with strontium ions with type I collagen, to formulate a bioactive ink for 3D-printing technologies. Moreover, to avoid the premature release of strontium ions within the crosslinking medium and to significantly increase the material mechanical and thermal stability, we applied an optimized chemical treatment using ethanol-dissolved genipin solutions. The high biocompatibility of the hybrid system was confirmed by using MG-63 and Saos-2 osteoblast-like cell lines, further highlighting the great potential of the innovative nanocomposite for the design of bone-like scaffolds. MDPI 2020-08-27 /pmc/articles/PMC7558137/ /pubmed/32867075 http://dx.doi.org/10.3390/nano10091681 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Montalbano, Giorgia
Borciani, Giorgia
Cerqueni, Giorgia
Licini, Caterina
Banche-Niclot, Federica
Janner, Davide
Sola, Stefania
Fiorilli, Sonia
Mattioli-Belmonte, Monica
Ciapetti, Gabriela
Vitale-Brovarone, Chiara
Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title_full Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title_fullStr Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title_full_unstemmed Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title_short Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy
title_sort collagen hybrid formulations for the 3d printing of nanostructured bone scaffolds: an optimized genipin-crosslinking strategy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558137/
https://www.ncbi.nlm.nih.gov/pubmed/32867075
http://dx.doi.org/10.3390/nano10091681
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