Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay

The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples...

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Autores principales: Graham, Maryza, Williams, Eloise, Isles, Nicole, Buadromo, Eka, Toatu, Tebuka, Druce, Julian, Catton, Mike, Lin, Chantel, Howden, Benjamin P., Williamson, Deborah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558241/
https://www.ncbi.nlm.nih.gov/pubmed/33171384
http://dx.doi.org/10.1016/j.diagmicrobio.2020.115238
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author Graham, Maryza
Williams, Eloise
Isles, Nicole
Buadromo, Eka
Toatu, Tebuka
Druce, Julian
Catton, Mike
Lin, Chantel
Howden, Benjamin P.
Williamson, Deborah A.
author_facet Graham, Maryza
Williams, Eloise
Isles, Nicole
Buadromo, Eka
Toatu, Tebuka
Druce, Julian
Catton, Mike
Lin, Chantel
Howden, Benjamin P.
Williamson, Deborah A.
author_sort Graham, Maryza
collection PubMed
description The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had ‘neat’ Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.
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spelling pubmed-75582412020-10-15 Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay Graham, Maryza Williams, Eloise Isles, Nicole Buadromo, Eka Toatu, Tebuka Druce, Julian Catton, Mike Lin, Chantel Howden, Benjamin P. Williamson, Deborah A. Diagn Microbiol Infect Dis Article The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had ‘neat’ Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity. The Authors. Published by Elsevier Inc. 2021-02 2020-10-15 /pmc/articles/PMC7558241/ /pubmed/33171384 http://dx.doi.org/10.1016/j.diagmicrobio.2020.115238 Text en © 2020 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Graham, Maryza
Williams, Eloise
Isles, Nicole
Buadromo, Eka
Toatu, Tebuka
Druce, Julian
Catton, Mike
Lin, Chantel
Howden, Benjamin P.
Williamson, Deborah A.
Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title_full Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title_fullStr Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title_full_unstemmed Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title_short Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
title_sort sample pooling on the cepheid xpert® xpress sars-cov-2 assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558241/
https://www.ncbi.nlm.nih.gov/pubmed/33171384
http://dx.doi.org/10.1016/j.diagmicrobio.2020.115238
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