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Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years

There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock cul...

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Autores principales: Lima, Soraia Lopes, Francisco, Elaine Cristina, de Almeida Júnior, João Nóbrega, Santos, Daniel Wagner de Castro Lima, Carlesse, Fabiane, Queiroz-Telles, Flávio, Melo, Analy Salles de Azevedo, Colombo, Arnaldo Lopes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558365/
https://www.ncbi.nlm.nih.gov/pubmed/32679832
http://dx.doi.org/10.3390/jof6030110
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author Lima, Soraia Lopes
Francisco, Elaine Cristina
de Almeida Júnior, João Nóbrega
Santos, Daniel Wagner de Castro Lima
Carlesse, Fabiane
Queiroz-Telles, Flávio
Melo, Analy Salles de Azevedo
Colombo, Arnaldo Lopes
author_facet Lima, Soraia Lopes
Francisco, Elaine Cristina
de Almeida Júnior, João Nóbrega
Santos, Daniel Wagner de Castro Lima
Carlesse, Fabiane
Queiroz-Telles, Flávio
Melo, Analy Salles de Azevedo
Colombo, Arnaldo Lopes
author_sort Lima, Soraia Lopes
collection PubMed
description There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock culture collected during the last 11 years. The isolates were identified by sequencing the rDNA internal transcribed spacer (ITS) region, and antifungal susceptibility tests for amphotericin B, voriconazole, fluconazole, anidulafungin, and 5-fluorocytosine were performed by the Clinical and Laboratory Standards Institute (CLSI) microbroth method. A total of 49 isolates were identified as Candida haemulonii sensu stricto (n = 21), followed by C. haemulonii var. vulnera (n = 15) and C. duobushaemulonii (n = 13), including 38 isolates cultured from patients with deep-seated Candida infections. The prevalence of the C. haemulonii species complex increased from 0.9% (18 isolates among 1931) in the first period (December 2008 to June 2013) to 1.7% (31 isolates among 1868) in the second period (July 2014 to December 2019) of analysis (p = 0.047). All isolates tested exhibited high minimum inhibition concentrations for amphotericin B and fluconazole, but they remained susceptible to 5-fluorocytosine and anidulafungin. We were able to demonstrate the increased isolation of the multiresistant Candida haemulonii species complex in our culture collection, where most isolates were cultured from patients with deep-seated infections.
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spelling pubmed-75583652020-10-22 Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years Lima, Soraia Lopes Francisco, Elaine Cristina de Almeida Júnior, João Nóbrega Santos, Daniel Wagner de Castro Lima Carlesse, Fabiane Queiroz-Telles, Flávio Melo, Analy Salles de Azevedo Colombo, Arnaldo Lopes J Fungi (Basel) Article There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock culture collected during the last 11 years. The isolates were identified by sequencing the rDNA internal transcribed spacer (ITS) region, and antifungal susceptibility tests for amphotericin B, voriconazole, fluconazole, anidulafungin, and 5-fluorocytosine were performed by the Clinical and Laboratory Standards Institute (CLSI) microbroth method. A total of 49 isolates were identified as Candida haemulonii sensu stricto (n = 21), followed by C. haemulonii var. vulnera (n = 15) and C. duobushaemulonii (n = 13), including 38 isolates cultured from patients with deep-seated Candida infections. The prevalence of the C. haemulonii species complex increased from 0.9% (18 isolates among 1931) in the first period (December 2008 to June 2013) to 1.7% (31 isolates among 1868) in the second period (July 2014 to December 2019) of analysis (p = 0.047). All isolates tested exhibited high minimum inhibition concentrations for amphotericin B and fluconazole, but they remained susceptible to 5-fluorocytosine and anidulafungin. We were able to demonstrate the increased isolation of the multiresistant Candida haemulonii species complex in our culture collection, where most isolates were cultured from patients with deep-seated infections. MDPI 2020-07-15 /pmc/articles/PMC7558365/ /pubmed/32679832 http://dx.doi.org/10.3390/jof6030110 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Soraia Lopes
Francisco, Elaine Cristina
de Almeida Júnior, João Nóbrega
Santos, Daniel Wagner de Castro Lima
Carlesse, Fabiane
Queiroz-Telles, Flávio
Melo, Analy Salles de Azevedo
Colombo, Arnaldo Lopes
Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title_full Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title_fullStr Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title_full_unstemmed Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title_short Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
title_sort increasing prevalence of multidrug-resistant candida haemulonii species complex among all yeast cultures collected by a reference laboratory over the past 11 years
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558365/
https://www.ncbi.nlm.nih.gov/pubmed/32679832
http://dx.doi.org/10.3390/jof6030110
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