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Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years
There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock cul...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558365/ https://www.ncbi.nlm.nih.gov/pubmed/32679832 http://dx.doi.org/10.3390/jof6030110 |
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author | Lima, Soraia Lopes Francisco, Elaine Cristina de Almeida Júnior, João Nóbrega Santos, Daniel Wagner de Castro Lima Carlesse, Fabiane Queiroz-Telles, Flávio Melo, Analy Salles de Azevedo Colombo, Arnaldo Lopes |
author_facet | Lima, Soraia Lopes Francisco, Elaine Cristina de Almeida Júnior, João Nóbrega Santos, Daniel Wagner de Castro Lima Carlesse, Fabiane Queiroz-Telles, Flávio Melo, Analy Salles de Azevedo Colombo, Arnaldo Lopes |
author_sort | Lima, Soraia Lopes |
collection | PubMed |
description | There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock culture collected during the last 11 years. The isolates were identified by sequencing the rDNA internal transcribed spacer (ITS) region, and antifungal susceptibility tests for amphotericin B, voriconazole, fluconazole, anidulafungin, and 5-fluorocytosine were performed by the Clinical and Laboratory Standards Institute (CLSI) microbroth method. A total of 49 isolates were identified as Candida haemulonii sensu stricto (n = 21), followed by C. haemulonii var. vulnera (n = 15) and C. duobushaemulonii (n = 13), including 38 isolates cultured from patients with deep-seated Candida infections. The prevalence of the C. haemulonii species complex increased from 0.9% (18 isolates among 1931) in the first period (December 2008 to June 2013) to 1.7% (31 isolates among 1868) in the second period (July 2014 to December 2019) of analysis (p = 0.047). All isolates tested exhibited high minimum inhibition concentrations for amphotericin B and fluconazole, but they remained susceptible to 5-fluorocytosine and anidulafungin. We were able to demonstrate the increased isolation of the multiresistant Candida haemulonii species complex in our culture collection, where most isolates were cultured from patients with deep-seated infections. |
format | Online Article Text |
id | pubmed-7558365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75583652020-10-22 Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years Lima, Soraia Lopes Francisco, Elaine Cristina de Almeida Júnior, João Nóbrega Santos, Daniel Wagner de Castro Lima Carlesse, Fabiane Queiroz-Telles, Flávio Melo, Analy Salles de Azevedo Colombo, Arnaldo Lopes J Fungi (Basel) Article There is worldwide concern with the increasing rates of infections due to multiresistant Candida isolates reported in tertiary medical centers. We checked for historical trends in terms of prevalence rates and antifungal susceptibility of the Candida haemulonii species complex in our yeast stock culture collected during the last 11 years. The isolates were identified by sequencing the rDNA internal transcribed spacer (ITS) region, and antifungal susceptibility tests for amphotericin B, voriconazole, fluconazole, anidulafungin, and 5-fluorocytosine were performed by the Clinical and Laboratory Standards Institute (CLSI) microbroth method. A total of 49 isolates were identified as Candida haemulonii sensu stricto (n = 21), followed by C. haemulonii var. vulnera (n = 15) and C. duobushaemulonii (n = 13), including 38 isolates cultured from patients with deep-seated Candida infections. The prevalence of the C. haemulonii species complex increased from 0.9% (18 isolates among 1931) in the first period (December 2008 to June 2013) to 1.7% (31 isolates among 1868) in the second period (July 2014 to December 2019) of analysis (p = 0.047). All isolates tested exhibited high minimum inhibition concentrations for amphotericin B and fluconazole, but they remained susceptible to 5-fluorocytosine and anidulafungin. We were able to demonstrate the increased isolation of the multiresistant Candida haemulonii species complex in our culture collection, where most isolates were cultured from patients with deep-seated infections. MDPI 2020-07-15 /pmc/articles/PMC7558365/ /pubmed/32679832 http://dx.doi.org/10.3390/jof6030110 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima, Soraia Lopes Francisco, Elaine Cristina de Almeida Júnior, João Nóbrega Santos, Daniel Wagner de Castro Lima Carlesse, Fabiane Queiroz-Telles, Flávio Melo, Analy Salles de Azevedo Colombo, Arnaldo Lopes Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title | Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title_full | Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title_fullStr | Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title_full_unstemmed | Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title_short | Increasing Prevalence of Multidrug-Resistant Candida haemulonii Species Complex among All Yeast Cultures Collected by a Reference Laboratory over the Past 11 Years |
title_sort | increasing prevalence of multidrug-resistant candida haemulonii species complex among all yeast cultures collected by a reference laboratory over the past 11 years |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558365/ https://www.ncbi.nlm.nih.gov/pubmed/32679832 http://dx.doi.org/10.3390/jof6030110 |
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