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High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation

A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9 positioning. Recently, two Cas9 variants with a relaxe...

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Detalles Bibliográficos
Autores principales:	Legut, Mateusz, Daniloski, Zharko, Xue, Xinhe, McKenzie, Dayna, Guo, Xinyi, Wessels, Hans-Hermann, Sanjana, Neville E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558435/ https://www.ncbi.nlm.nih.gov/pubmed/32130891 http://dx.doi.org/10.1016/j.celrep.2020.02.010

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