Cargando…

Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens

BACKGROUND: Pooled library CRISPR/Cas9 knockout screening across hundreds of cell lines has identified genes whose disruption leads to fitness defects, a critical step in identifying candidate cancer targets. However, the number of essential genes detected from these monogenic knockout screens is lo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dede, Merve, McLaughlin, Megan, Kim, Eiru, Hart, Traver
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558751/ https://www.ncbi.nlm.nih.gov/pubmed/33059726 http://dx.doi.org/10.1186/s13059-020-02173-2

Ejemplares similares

A network of human functional gene interactions from knockout fitness screens in cancer cells
por: Kim, Eiru, et al.
Publicado: (2019)

Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform
por: Anvar, Nazanin Esmaeili, et al.
Publicado: (2023)

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
por: Hart, Traver, et al.
Publicado: (2017)

JACKS: joint analysis of CRISPR/Cas9 knockout screens
por: Allen, Felicity, et al.
Publicado: (2019)

Recovering false negatives in CRISPR fitness screens with JLOE
por: Dede, Merve, et al.
Publicado: (2023)

Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
por: Patel, Lalit R., et al.
Publicado: (2023)

Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls
por: Sanor, Lucas D, et al.
Publicado: (2020)

Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
por: Estep, Jason A., et al.
Publicado: (2016)

Improved analysis of CRISPR fitness screens and reduced off-target effects with the BAGEL2 gene essentiality classifier
por: Kim, Eiru, et al.
Publicado: (2021)

Genome-scale CRISPR-Cas9 knockout screening in hepatocellular carcinoma with lenvatinib resistance
por: Lu, Yonggang, et al.
Publicado: (2021)

Discovery of putative tumor suppressors from CRISPR screens reveals rewired lipid metabolism in acute myeloid leukemia cells
por: Lenoir, W. Frank, et al.
Publicado: (2021)

Multiplexed Knockouts in the Model Diatom Phaeodactylum by Episomal Delivery of a Selectable Cas9
por: Moosburner, Mark Andrew, et al.
Publicado: (2020)

Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora
por: Casarin, Tatiane, et al.
Publicado: (2022)

Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
por: Park, Jeongbin, et al.
Publicado: (2016)

Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance
por: Cao, Jie, et al.
Publicado: (2018)

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation
por: Legut, Mateusz, et al.
Publicado: (2020)

Correction: Genome-scale CRISPR-Cas9 knockout screening in hepatocellular carcinoma with lenvatinib resistance
por: Lu, Yonggang, et al.
Publicado: (2022)

Genome-scale CRISPR-Cas9 knockout screening in nasopharyngeal carcinoma for radiosensitive and radioresistant genes
por: Zhou, Ziyan, et al.
Publicado: (2023)

CRISPR-Cas9 knockout screening identifies KIAA1429 as an essential gene in Ewing sarcoma
por: Tan, Kezhe, et al.
Publicado: (2023)

Human Monogenic Disease Genes Have Frequently Functionally Redundant Paralogs
por: Chen, Wei-Hua, et al.
Publicado: (2013)

CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma
por: Wenger, Anna, et al.
Publicado: (2023)

Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
por: Khan, Fehad J., et al.
Publicado: (2019)

Novel disease resistance gene paralogs created by CRISPR/Cas9 in soy
por: Nagy, Ervin D., et al.
Publicado: (2021)

CRISPR/Cas9-induced gene conversion between ATAD3 paralogs
por: Yanovsky-Dagan, Shira, et al.
Publicado: (2022)

Genomic screening for monogenic forms of diabetes
por: Biesecker, Leslie G.
Publicado: (2018)

Using CRISPR/Cas9-Mediated GLA Gene Knockout as an In Vitro Drug Screening Model for Fabry Disease
por: Song, Hui-Yung, et al.
Publicado: (2016)

In vivo CRISPR/Cas9 knockout screen: TCEAL1 silencing enhances docetaxel efficacy in prostate cancer
por: Rushworth, Linda K, et al.
Publicado: (2020)

Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout
por: Yang, Bin, et al.
Publicado: (2020)

BaSDAS: a web-based pooled CRISPR-Cas9 knockout screening data analysis system
por: Park, Young-Kyu, et al.
Publicado: (2020)

Kinome-wide CRISPR-Cas9 knockout screens revealed PLK1 as a therapeutic target for osteosarcoma
por: Wang, Renxian, et al.
Publicado: (2023)

PICKLES: the database of pooled in-vitro CRISPR knockout library essentiality screens
por: Lenoir, Walter F, et al.
Publicado: (2018)

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens
por: Li, Wei, et al.
Publicado: (2014)

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
por: He, Wei, et al.
Publicado: (2019)

Multiplexed CRISPR/Cas9-Mediated Knockout of Laccase Genes in Salvia miltiorrhiza Revealed Their Roles in Growth, Development, and Metabolism
por: Zhou, Zheng, et al.
Publicado: (2021)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

A genome-scale CRISPR/Cas9 knockout screening reveals SH3D21 as a sensitizer for gemcitabine
por: Masoudi, Mohammad, et al.
Publicado: (2019)

PB1976: CRISPR-CAS9 KNOCKOUT SCREEN IDENTIFIES RNA MODIFICATION ENZYMES ESSENTIAL FOR CELL SURVIVAL IN MULTIPLE MYELOMA
por: Zhao, D., et al.
Publicado: (2022)

Establishment of a CRISPR/Cas9 knockout library for screening type I interferon-inducible antiviral effectors in pig cells
por: Dang, Wen, et al.
Publicado: (2022)

A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase
por: Jin, Da-Yun, et al.
Publicado: (2023)

Yeast genetic interaction screens in the age of CRISPR/Cas
por: Adames, Neil R., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_j85kl2vf4eimbnot4ptlgf1emt