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Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens

BACKGROUND: Pooled library CRISPR/Cas9 knockout screening across hundreds of cell lines has identified genes whose disruption leads to fitness defects, a critical step in identifying candidate cancer targets. However, the number of essential genes detected from these monogenic knockout screens is lo...

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Detalles Bibliográficos
Autores principales:	Dede, Merve, McLaughlin, Megan, Kim, Eiru, Hart, Traver
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558751/ https://www.ncbi.nlm.nih.gov/pubmed/33059726 http://dx.doi.org/10.1186/s13059-020-02173-2

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