Cargando…

Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production techn...

Descripción completa

Detalles Bibliográficos
Autores principales: Suntara, Chanon, Cherdthong, Anusorn, Uriyapongson, Suthipong, Wanapat, Metha, Chanjula, Pin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558849/
https://www.ncbi.nlm.nih.gov/pubmed/32679708
http://dx.doi.org/10.3390/jof6030109
_version_ 1783594727443005440
author Suntara, Chanon
Cherdthong, Anusorn
Uriyapongson, Suthipong
Wanapat, Metha
Chanjula, Pin
author_facet Suntara, Chanon
Cherdthong, Anusorn
Uriyapongson, Suthipong
Wanapat, Metha
Chanjula, Pin
author_sort Suntara, Chanon
collection PubMed
description The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.
format Online
Article
Text
id pubmed-7558849
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75588492020-10-26 Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production Suntara, Chanon Cherdthong, Anusorn Uriyapongson, Suthipong Wanapat, Metha Chanjula, Pin J Fungi (Basel) Article The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility. MDPI 2020-07-15 /pmc/articles/PMC7558849/ /pubmed/32679708 http://dx.doi.org/10.3390/jof6030109 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suntara, Chanon
Cherdthong, Anusorn
Uriyapongson, Suthipong
Wanapat, Metha
Chanjula, Pin
Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title_full Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title_fullStr Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title_full_unstemmed Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title_short Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production
title_sort comparison effects of ruminal crabtree-negative yeasts and crabtree-positive yeasts for improving ensiled rice straw quality and ruminal digestion using in vitro gas production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558849/
https://www.ncbi.nlm.nih.gov/pubmed/32679708
http://dx.doi.org/10.3390/jof6030109
work_keys_str_mv AT suntarachanon comparisoneffectsofruminalcrabtreenegativeyeastsandcrabtreepositiveyeastsforimprovingensiledricestrawqualityandruminaldigestionusinginvitrogasproduction
AT cherdthonganusorn comparisoneffectsofruminalcrabtreenegativeyeastsandcrabtreepositiveyeastsforimprovingensiledricestrawqualityandruminaldigestionusinginvitrogasproduction
AT uriyapongsonsuthipong comparisoneffectsofruminalcrabtreenegativeyeastsandcrabtreepositiveyeastsforimprovingensiledricestrawqualityandruminaldigestionusinginvitrogasproduction
AT wanapatmetha comparisoneffectsofruminalcrabtreenegativeyeastsandcrabtreepositiveyeastsforimprovingensiledricestrawqualityandruminaldigestionusinginvitrogasproduction
AT chanjulapin comparisoneffectsofruminalcrabtreenegativeyeastsandcrabtreepositiveyeastsforimprovingensiledricestrawqualityandruminaldigestionusinginvitrogasproduction