Cargando…

Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7

Monascus pigments (MPs) have been used as food colorants for several centuries in Asian countries and are now used throughout the world via Asian catering. The MP biosynthetic pathway has been well-illustrated, but the functions of a few genes, including mrpigG, in the MP gene cluster are still uncl...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Li, Chen, Fusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558996/
https://www.ncbi.nlm.nih.gov/pubmed/32872515
http://dx.doi.org/10.3390/jof6030156
_version_ 1783594760344174592
author Li, Li
Chen, Fusheng
author_facet Li, Li
Chen, Fusheng
author_sort Li, Li
collection PubMed
description Monascus pigments (MPs) have been used as food colorants for several centuries in Asian countries and are now used throughout the world via Asian catering. The MP biosynthetic pathway has been well-illustrated, but the functions of a few genes, including mrpigG, in the MP gene cluster are still unclear. In the current study, in order to investigate the function of mrpigG in M. ruber M7, gene deletion (ΔmrpigG), complementation (ΔmrpigG::mrpigG) and overexpression (M7::PtrpC-mrpigG) mutants were successfully obtained. The morphologies and biomasses, as well as the MP and citrinin production, of these mutants were analyzed. The results revealed that the disruption, complementation and overexpression of mrpigG showed no apparent defects in morphology, biomass or citrinin production (except MP production) in ΔmrpigG compared with M. ruber M7. Although the MP profiles of ΔmrpigG and M. ruber M7 were almost the same—with both having four yellow pigments, two orange pigments (OPs) and two red pigments (RPs)—their yields were decreased in ΔmrpigG to a certain extent. Particularly, the content of rubropunctatin (an OP) and its derivative rubropunctamine (an RP) in ΔmrpigG, both of which have a five-carbon side chain, accounted for 57.7%, and 22.3% of those in M. ruber M7. On the other hand, monascorubrin (an OP) and its derivative monascorubramine (an RP), both of which have a seven-carbon side chain, were increased by 1.15 and 2.55 times, respectively, in ΔmrpigG compared with M. ruber M7. These results suggest that the MrPigG protein may preferentially catalyze the biosynthesis of MPs with a five-carbon side chain.
format Online
Article
Text
id pubmed-7558996
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75589962020-10-26 Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7 Li, Li Chen, Fusheng J Fungi (Basel) Article Monascus pigments (MPs) have been used as food colorants for several centuries in Asian countries and are now used throughout the world via Asian catering. The MP biosynthetic pathway has been well-illustrated, but the functions of a few genes, including mrpigG, in the MP gene cluster are still unclear. In the current study, in order to investigate the function of mrpigG in M. ruber M7, gene deletion (ΔmrpigG), complementation (ΔmrpigG::mrpigG) and overexpression (M7::PtrpC-mrpigG) mutants were successfully obtained. The morphologies and biomasses, as well as the MP and citrinin production, of these mutants were analyzed. The results revealed that the disruption, complementation and overexpression of mrpigG showed no apparent defects in morphology, biomass or citrinin production (except MP production) in ΔmrpigG compared with M. ruber M7. Although the MP profiles of ΔmrpigG and M. ruber M7 were almost the same—with both having four yellow pigments, two orange pigments (OPs) and two red pigments (RPs)—their yields were decreased in ΔmrpigG to a certain extent. Particularly, the content of rubropunctatin (an OP) and its derivative rubropunctamine (an RP) in ΔmrpigG, both of which have a five-carbon side chain, accounted for 57.7%, and 22.3% of those in M. ruber M7. On the other hand, monascorubrin (an OP) and its derivative monascorubramine (an RP), both of which have a seven-carbon side chain, were increased by 1.15 and 2.55 times, respectively, in ΔmrpigG compared with M. ruber M7. These results suggest that the MrPigG protein may preferentially catalyze the biosynthesis of MPs with a five-carbon side chain. MDPI 2020-08-29 /pmc/articles/PMC7558996/ /pubmed/32872515 http://dx.doi.org/10.3390/jof6030156 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Li
Chen, Fusheng
Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title_full Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title_fullStr Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title_full_unstemmed Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title_short Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
title_sort effects of mrpigg on development and secondary metabolism of monascus ruber m7
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558996/
https://www.ncbi.nlm.nih.gov/pubmed/32872515
http://dx.doi.org/10.3390/jof6030156
work_keys_str_mv AT lili effectsofmrpiggondevelopmentandsecondarymetabolismofmonascusruberm7
AT chenfusheng effectsofmrpiggondevelopmentandsecondarymetabolismofmonascusruberm7