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Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica

Puumala virus (PUUV) in Europe causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). The incidence of NE is highly heterogeneous spatially, whereas the geographic distribution of the wild reservoir of PUUV, the bank vole, is essentially homogeneous. Our und...

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Autores principales: Murri, Séverine, Madrières, Sarah, Tatard, Caroline, Piry, Sylvain, Benoit, Laure, Loiseau, Anne, Pradel, Julien, Artige, Emmanuelle, Audiot, Philippe, Leménager, Nicolas, Lacôte, Sandra, Vulin, Johann, Charbonnel, Nathalie, Marianneau, Philippe, Castel, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559001/
https://www.ncbi.nlm.nih.gov/pubmed/32882953
http://dx.doi.org/10.3390/pathogens9090721
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author Murri, Séverine
Madrières, Sarah
Tatard, Caroline
Piry, Sylvain
Benoit, Laure
Loiseau, Anne
Pradel, Julien
Artige, Emmanuelle
Audiot, Philippe
Leménager, Nicolas
Lacôte, Sandra
Vulin, Johann
Charbonnel, Nathalie
Marianneau, Philippe
Castel, Guillaume
author_facet Murri, Séverine
Madrières, Sarah
Tatard, Caroline
Piry, Sylvain
Benoit, Laure
Loiseau, Anne
Pradel, Julien
Artige, Emmanuelle
Audiot, Philippe
Leménager, Nicolas
Lacôte, Sandra
Vulin, Johann
Charbonnel, Nathalie
Marianneau, Philippe
Castel, Guillaume
author_sort Murri, Séverine
collection PubMed
description Puumala virus (PUUV) in Europe causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). The incidence of NE is highly heterogeneous spatially, whereas the geographic distribution of the wild reservoir of PUUV, the bank vole, is essentially homogeneous. Our understanding of the processes driving this heterogeneity remains incomplete due to gaps in knowledge. Little is known about the current distribution and genetic variation of PUUV in the areas outside the well-identified zones of NE endemicity. We trapped bank voles in four forests in French regions in which NE is considered non-endemic, but sporadic NE cases have been reported recently. We tested bank voles for anti-PUUV IgG and characterized the S segment sequences of PUUV from seropositive animals. Phylogenetic analyses revealed specific amino-acid signatures and genetic differences between PUUV circulating in non-endemic and nearby NE-endemic areas. We also showed, in temporal surveys, that the amino-acid sequences of PUUV had undergone fewer recent changes in areas non-endemic for NE than in endemic areas. The evolutionary history of the current French PUUV clusters was investigated by phylogeographic approaches, and the results were considered in the context of the history of French forests. Our findings highlight the need to monitor the circulation and genetics of PUUV in a larger array of bank vole populations, to improve our understanding of the risk of NE.
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spelling pubmed-75590012020-10-26 Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica Murri, Séverine Madrières, Sarah Tatard, Caroline Piry, Sylvain Benoit, Laure Loiseau, Anne Pradel, Julien Artige, Emmanuelle Audiot, Philippe Leménager, Nicolas Lacôte, Sandra Vulin, Johann Charbonnel, Nathalie Marianneau, Philippe Castel, Guillaume Pathogens Article Puumala virus (PUUV) in Europe causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). The incidence of NE is highly heterogeneous spatially, whereas the geographic distribution of the wild reservoir of PUUV, the bank vole, is essentially homogeneous. Our understanding of the processes driving this heterogeneity remains incomplete due to gaps in knowledge. Little is known about the current distribution and genetic variation of PUUV in the areas outside the well-identified zones of NE endemicity. We trapped bank voles in four forests in French regions in which NE is considered non-endemic, but sporadic NE cases have been reported recently. We tested bank voles for anti-PUUV IgG and characterized the S segment sequences of PUUV from seropositive animals. Phylogenetic analyses revealed specific amino-acid signatures and genetic differences between PUUV circulating in non-endemic and nearby NE-endemic areas. We also showed, in temporal surveys, that the amino-acid sequences of PUUV had undergone fewer recent changes in areas non-endemic for NE than in endemic areas. The evolutionary history of the current French PUUV clusters was investigated by phylogeographic approaches, and the results were considered in the context of the history of French forests. Our findings highlight the need to monitor the circulation and genetics of PUUV in a larger array of bank vole populations, to improve our understanding of the risk of NE. MDPI 2020-09-01 /pmc/articles/PMC7559001/ /pubmed/32882953 http://dx.doi.org/10.3390/pathogens9090721 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murri, Séverine
Madrières, Sarah
Tatard, Caroline
Piry, Sylvain
Benoit, Laure
Loiseau, Anne
Pradel, Julien
Artige, Emmanuelle
Audiot, Philippe
Leménager, Nicolas
Lacôte, Sandra
Vulin, Johann
Charbonnel, Nathalie
Marianneau, Philippe
Castel, Guillaume
Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title_full Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title_fullStr Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title_full_unstemmed Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title_short Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica
title_sort detection and genetic characterization of puumala orthohantavirus s-segment in areas of france non-endemic for nephropathia epidemica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559001/
https://www.ncbi.nlm.nih.gov/pubmed/32882953
http://dx.doi.org/10.3390/pathogens9090721
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