Cargando…
Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin
Mucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N′-(di-m-methylphenyl)ure...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559021/ https://www.ncbi.nlm.nih.gov/pubmed/32731584 http://dx.doi.org/10.3390/biomimetics5030036 |
_version_ | 1783594765493731328 |
---|---|
author | Collado-González, Mar Kaur, Gurmeet González-Espinosa, Yadira Brooks, Rebecca Goycoolea, Francisco M. |
author_facet | Collado-González, Mar Kaur, Gurmeet González-Espinosa, Yadira Brooks, Rebecca Goycoolea, Francisco M. |
author_sort | Collado-González, Mar |
collection | PubMed |
description | Mucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N′-(di-m-methylphenyl)urea (DMTU), a quorum-sensing inhibitor, to the oral cavity. DMTU-loaded NCs constitute an alternative for the treatment of plaque (bacterial biofilm). In this work, the stability of the NCs after their interaction with mucin is analysed. Mucin type III from Sigma-Aldrich has been used as the mucin model. Mucin and NCs were characterised by the multi-detection asymmetrical flow field-flow fractionation technique (AF4). Dynamic light scattering (DLS) and ζ-potential analyses were carried out to characterise the interaction between mucin and NCs. According to the results, loading DMTU changes the conformation of the NC. It was also found that the synergistic interaction between mucin and NCs was favoured within a specific range of the mucin:NC ratio within the first 24 h. Studies on the release of DMTU in vitro and the microbial activity of such NCs are ongoing in our lab. |
format | Online Article Text |
id | pubmed-7559021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75590212020-10-26 Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin Collado-González, Mar Kaur, Gurmeet González-Espinosa, Yadira Brooks, Rebecca Goycoolea, Francisco M. Biomimetics (Basel) Article Mucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N′-(di-m-methylphenyl)urea (DMTU), a quorum-sensing inhibitor, to the oral cavity. DMTU-loaded NCs constitute an alternative for the treatment of plaque (bacterial biofilm). In this work, the stability of the NCs after their interaction with mucin is analysed. Mucin type III from Sigma-Aldrich has been used as the mucin model. Mucin and NCs were characterised by the multi-detection asymmetrical flow field-flow fractionation technique (AF4). Dynamic light scattering (DLS) and ζ-potential analyses were carried out to characterise the interaction between mucin and NCs. According to the results, loading DMTU changes the conformation of the NC. It was also found that the synergistic interaction between mucin and NCs was favoured within a specific range of the mucin:NC ratio within the first 24 h. Studies on the release of DMTU in vitro and the microbial activity of such NCs are ongoing in our lab. MDPI 2020-07-28 /pmc/articles/PMC7559021/ /pubmed/32731584 http://dx.doi.org/10.3390/biomimetics5030036 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Collado-González, Mar Kaur, Gurmeet González-Espinosa, Yadira Brooks, Rebecca Goycoolea, Francisco M. Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title | Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title_full | Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title_fullStr | Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title_full_unstemmed | Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title_short | Characterisation of the Interaction among Oil-In-Water Nanocapsules and Mucin |
title_sort | characterisation of the interaction among oil-in-water nanocapsules and mucin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559021/ https://www.ncbi.nlm.nih.gov/pubmed/32731584 http://dx.doi.org/10.3390/biomimetics5030036 |
work_keys_str_mv | AT colladogonzalezmar characterisationoftheinteractionamongoilinwaternanocapsulesandmucin AT kaurgurmeet characterisationoftheinteractionamongoilinwaternanocapsulesandmucin AT gonzalezespinosayadira characterisationoftheinteractionamongoilinwaternanocapsulesandmucin AT brooksrebecca characterisationoftheinteractionamongoilinwaternanocapsulesandmucin AT goycooleafranciscom characterisationoftheinteractionamongoilinwaternanocapsulesandmucin |