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Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection

Field effect transistors (FETs) based on networks of randomly oriented Si nanowires (Si nanonets or Si NNs) were biomodified using Thrombin Binding Aptamer (TBA–15) probe with the final objective to sense thrombin by electrical detection. In this work, the impact of the biomodification on the electr...

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Autores principales: Vallejo-Perez, Monica, Ternon, Céline, Spinelli, Nicolas, Morisot, Fanny, Theodorou, Christoforos, Jayakumar, Ganesh, Hellström, Per-Erik, Mouis, Mireille, Rapenne, Laetitia, Mescot, Xavier, Salem, Bassem, Stambouli, Valérie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559082/
https://www.ncbi.nlm.nih.gov/pubmed/32942692
http://dx.doi.org/10.3390/nano10091842
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author Vallejo-Perez, Monica
Ternon, Céline
Spinelli, Nicolas
Morisot, Fanny
Theodorou, Christoforos
Jayakumar, Ganesh
Hellström, Per-Erik
Mouis, Mireille
Rapenne, Laetitia
Mescot, Xavier
Salem, Bassem
Stambouli, Valérie
author_facet Vallejo-Perez, Monica
Ternon, Céline
Spinelli, Nicolas
Morisot, Fanny
Theodorou, Christoforos
Jayakumar, Ganesh
Hellström, Per-Erik
Mouis, Mireille
Rapenne, Laetitia
Mescot, Xavier
Salem, Bassem
Stambouli, Valérie
author_sort Vallejo-Perez, Monica
collection PubMed
description Field effect transistors (FETs) based on networks of randomly oriented Si nanowires (Si nanonets or Si NNs) were biomodified using Thrombin Binding Aptamer (TBA–15) probe with the final objective to sense thrombin by electrical detection. In this work, the impact of the biomodification on the electrical properties of the Si NN–FETs was studied. First, the results that were obtained for the optimization of the (3-Glycidyloxypropyl)trimethoxysilane (GOPS)-based biofunctionalization process by using UV radiation are reported. The biofunctionalized devices were analyzed by atomic force microscopy (AFM) and scanning transmission electron microscopy (STEM), proving that TBA–15 probes were properly grafted on the surface of the devices, and by means of epifluorescence microscopy it was possible to demonstrate that the UV-assisted GOPS-based functionalization notably improves the homogeneity of the surface DNA distribution. Later, the electrical characteristics of 80 devices were analyzed before and after the biofunctionalization process, indicating that the results are highly dependent on the experimental protocol. We found that the TBA–15 hybridization capacity with its complementary strand is time dependent and that the transfer characteristics of the Si NN–FETs obtained after the TBA–15 probe grafting are also time dependent. These results help to elucidate and define the experimental precautions that must be taken into account to fabricate reproducible devices.
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spelling pubmed-75590822020-10-29 Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection Vallejo-Perez, Monica Ternon, Céline Spinelli, Nicolas Morisot, Fanny Theodorou, Christoforos Jayakumar, Ganesh Hellström, Per-Erik Mouis, Mireille Rapenne, Laetitia Mescot, Xavier Salem, Bassem Stambouli, Valérie Nanomaterials (Basel) Article Field effect transistors (FETs) based on networks of randomly oriented Si nanowires (Si nanonets or Si NNs) were biomodified using Thrombin Binding Aptamer (TBA–15) probe with the final objective to sense thrombin by electrical detection. In this work, the impact of the biomodification on the electrical properties of the Si NN–FETs was studied. First, the results that were obtained for the optimization of the (3-Glycidyloxypropyl)trimethoxysilane (GOPS)-based biofunctionalization process by using UV radiation are reported. The biofunctionalized devices were analyzed by atomic force microscopy (AFM) and scanning transmission electron microscopy (STEM), proving that TBA–15 probes were properly grafted on the surface of the devices, and by means of epifluorescence microscopy it was possible to demonstrate that the UV-assisted GOPS-based functionalization notably improves the homogeneity of the surface DNA distribution. Later, the electrical characteristics of 80 devices were analyzed before and after the biofunctionalization process, indicating that the results are highly dependent on the experimental protocol. We found that the TBA–15 hybridization capacity with its complementary strand is time dependent and that the transfer characteristics of the Si NN–FETs obtained after the TBA–15 probe grafting are also time dependent. These results help to elucidate and define the experimental precautions that must be taken into account to fabricate reproducible devices. MDPI 2020-09-15 /pmc/articles/PMC7559082/ /pubmed/32942692 http://dx.doi.org/10.3390/nano10091842 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vallejo-Perez, Monica
Ternon, Céline
Spinelli, Nicolas
Morisot, Fanny
Theodorou, Christoforos
Jayakumar, Ganesh
Hellström, Per-Erik
Mouis, Mireille
Rapenne, Laetitia
Mescot, Xavier
Salem, Bassem
Stambouli, Valérie
Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title_full Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title_fullStr Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title_full_unstemmed Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title_short Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
title_sort optimization of gops-based functionalization process and impact of aptamer grafting on the si nanonet fet electrical properties as first steps towards thrombin electrical detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559082/
https://www.ncbi.nlm.nih.gov/pubmed/32942692
http://dx.doi.org/10.3390/nano10091842
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