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Aptasensors for Point-of-Care Detection of Small Molecules

Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, inc...

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Autores principales: Prante, Marc, Segal, Ester, Scheper, Thomas, Bahnemann, Janina, Walter, Johanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559136/
https://www.ncbi.nlm.nih.gov/pubmed/32859075
http://dx.doi.org/10.3390/bios10090108
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author Prante, Marc
Segal, Ester
Scheper, Thomas
Bahnemann, Janina
Walter, Johanna
author_facet Prante, Marc
Segal, Ester
Scheper, Thomas
Bahnemann, Janina
Walter, Johanna
author_sort Prante, Marc
collection PubMed
description Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.
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spelling pubmed-75591362020-10-29 Aptasensors for Point-of-Care Detection of Small Molecules Prante, Marc Segal, Ester Scheper, Thomas Bahnemann, Janina Walter, Johanna Biosensors (Basel) Review Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented. MDPI 2020-08-26 /pmc/articles/PMC7559136/ /pubmed/32859075 http://dx.doi.org/10.3390/bios10090108 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Prante, Marc
Segal, Ester
Scheper, Thomas
Bahnemann, Janina
Walter, Johanna
Aptasensors for Point-of-Care Detection of Small Molecules
title Aptasensors for Point-of-Care Detection of Small Molecules
title_full Aptasensors for Point-of-Care Detection of Small Molecules
title_fullStr Aptasensors for Point-of-Care Detection of Small Molecules
title_full_unstemmed Aptasensors for Point-of-Care Detection of Small Molecules
title_short Aptasensors for Point-of-Care Detection of Small Molecules
title_sort aptasensors for point-of-care detection of small molecules
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559136/
https://www.ncbi.nlm.nih.gov/pubmed/32859075
http://dx.doi.org/10.3390/bios10090108
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