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Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua

In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from hous...

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Autores principales: Flores, Byron, Escobar, Karla, Muzquiz, José Luis, Sheleby-Elías, Jessica, Mora, Brenda, Roque, Edipcia, Torres, Dayana, Chávez, Álvaro, Jirón, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559144/
https://www.ncbi.nlm.nih.gov/pubmed/32962119
http://dx.doi.org/10.3390/tropicalmed5030149
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author Flores, Byron
Escobar, Karla
Muzquiz, José Luis
Sheleby-Elías, Jessica
Mora, Brenda
Roque, Edipcia
Torres, Dayana
Chávez, Álvaro
Jirón, William
author_facet Flores, Byron
Escobar, Karla
Muzquiz, José Luis
Sheleby-Elías, Jessica
Mora, Brenda
Roque, Edipcia
Torres, Dayana
Chávez, Álvaro
Jirón, William
author_sort Flores, Byron
collection PubMed
description In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases. Leptospira spp was isolated in Ellinghausen–McCullough–Johnson–Harris (EMJH) culture medium with 5-fluororacil and positive samples were analyzed through PCR for the LipL32 gene, specific for pathogenic leptospires (P1 clade). There were 73 positive cultures from 120 samples, however only six of these (5% of all collected samples) were confirmed to be pathogenic, based on the presence of the LipL32 gene (P1 clade). Of these six pathogenic isolates, four were from Leon and two from Chinandega. Four pathogenic isolates were obtained from water and two from soil. This study proved the contamination of water and soil with pathogenic leptospires, which represents a potential risk for public health.
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spelling pubmed-75591442020-10-29 Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua Flores, Byron Escobar, Karla Muzquiz, José Luis Sheleby-Elías, Jessica Mora, Brenda Roque, Edipcia Torres, Dayana Chávez, Álvaro Jirón, William Trop Med Infect Dis Article In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases. Leptospira spp was isolated in Ellinghausen–McCullough–Johnson–Harris (EMJH) culture medium with 5-fluororacil and positive samples were analyzed through PCR for the LipL32 gene, specific for pathogenic leptospires (P1 clade). There were 73 positive cultures from 120 samples, however only six of these (5% of all collected samples) were confirmed to be pathogenic, based on the presence of the LipL32 gene (P1 clade). Of these six pathogenic isolates, four were from Leon and two from Chinandega. Four pathogenic isolates were obtained from water and two from soil. This study proved the contamination of water and soil with pathogenic leptospires, which represents a potential risk for public health. MDPI 2020-09-18 /pmc/articles/PMC7559144/ /pubmed/32962119 http://dx.doi.org/10.3390/tropicalmed5030149 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Flores, Byron
Escobar, Karla
Muzquiz, José Luis
Sheleby-Elías, Jessica
Mora, Brenda
Roque, Edipcia
Torres, Dayana
Chávez, Álvaro
Jirón, William
Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title_full Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title_fullStr Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title_full_unstemmed Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title_short Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
title_sort detection of pathogenic leptospires in water and soil in areas endemic to leptospirosis in nicaragua
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559144/
https://www.ncbi.nlm.nih.gov/pubmed/32962119
http://dx.doi.org/10.3390/tropicalmed5030149
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