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SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion
Small nucleolar RNAs (snoRNAs) play a crucial role during colorectal cancer (CRC) development. The study of SNORA71A is few, and its role in CRC is unknown. This study focused on screening abnormal snoRNAs in CRC and exploring the role of key snoRNA in CRC. The expression pattern of snoRNAs in 3 CRC...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559222/ https://www.ncbi.nlm.nih.gov/pubmed/33083486 http://dx.doi.org/10.1155/2020/8284576 |
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author | Zhang, Zhengxiang Tao, Yunxiang Hua, Qingling Cai, Juan Ye, Xiaobing Li, Hao |
author_facet | Zhang, Zhengxiang Tao, Yunxiang Hua, Qingling Cai, Juan Ye, Xiaobing Li, Hao |
author_sort | Zhang, Zhengxiang |
collection | PubMed |
description | Small nucleolar RNAs (snoRNAs) play a crucial role during colorectal cancer (CRC) development. The study of SNORA71A is few, and its role in CRC is unknown. This study focused on screening abnormal snoRNAs in CRC and exploring the role of key snoRNA in CRC. The expression pattern of snoRNAs in 3 CRC and 3 normal colon tissues was detected via small RNA sequencing. The six candidate snoRNAs were identified by quantitative PCR (qPCR). Subsequently, the expression level of SNORA71A was further verified through the Cancer Genome Atlas (TCGA) data analysis and qPCR. The CCK8 and transwell assays were used to detect the functional role of SNORA71A in CRC cells. The integrated analysis of snoRNA expression profile indicated that a total 107 snoRNAs were significantly differentially expressed (DE) in CRC tissues compared with normal tissues, including 45 upregulated and 62 downregulated snoRNAs. Bioinformatics analysis revealed that the DE snoRNAs were mainly implicated in “detection of chemical stimulus involved in sensory perception of smell” and “sensory perception of smell” in the biological process. The DE snoRNAs were preferentially enriched in “olfactory transduction” and “glycosphingolipid biosynthesis-ganglio series pathway.” The expression of SNORA71A was upregulated in CRC tissues and cells. SNORA71A expression showed statistically significant correlations with TNM stage (P = 0.0196) and lymph node metastasis (P = 0.0189) and can serve as biomarkers for CRC. Importantly, SNORA71A significantly facilitated the CRC cell proliferation, migration, and invasion. Our findings indicate that SNORA71A screened by sequencing acted as an oncogene and promoted proliferation, migration, and invasion ability of CRC cells. |
format | Online Article Text |
id | pubmed-7559222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75592222020-10-19 SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion Zhang, Zhengxiang Tao, Yunxiang Hua, Qingling Cai, Juan Ye, Xiaobing Li, Hao Biomed Res Int Research Article Small nucleolar RNAs (snoRNAs) play a crucial role during colorectal cancer (CRC) development. The study of SNORA71A is few, and its role in CRC is unknown. This study focused on screening abnormal snoRNAs in CRC and exploring the role of key snoRNA in CRC. The expression pattern of snoRNAs in 3 CRC and 3 normal colon tissues was detected via small RNA sequencing. The six candidate snoRNAs were identified by quantitative PCR (qPCR). Subsequently, the expression level of SNORA71A was further verified through the Cancer Genome Atlas (TCGA) data analysis and qPCR. The CCK8 and transwell assays were used to detect the functional role of SNORA71A in CRC cells. The integrated analysis of snoRNA expression profile indicated that a total 107 snoRNAs were significantly differentially expressed (DE) in CRC tissues compared with normal tissues, including 45 upregulated and 62 downregulated snoRNAs. Bioinformatics analysis revealed that the DE snoRNAs were mainly implicated in “detection of chemical stimulus involved in sensory perception of smell” and “sensory perception of smell” in the biological process. The DE snoRNAs were preferentially enriched in “olfactory transduction” and “glycosphingolipid biosynthesis-ganglio series pathway.” The expression of SNORA71A was upregulated in CRC tissues and cells. SNORA71A expression showed statistically significant correlations with TNM stage (P = 0.0196) and lymph node metastasis (P = 0.0189) and can serve as biomarkers for CRC. Importantly, SNORA71A significantly facilitated the CRC cell proliferation, migration, and invasion. Our findings indicate that SNORA71A screened by sequencing acted as an oncogene and promoted proliferation, migration, and invasion ability of CRC cells. Hindawi 2020-10-05 /pmc/articles/PMC7559222/ /pubmed/33083486 http://dx.doi.org/10.1155/2020/8284576 Text en Copyright © 2020 Zhengxiang Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Zhengxiang Tao, Yunxiang Hua, Qingling Cai, Juan Ye, Xiaobing Li, Hao SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title | SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title_full | SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title_fullStr | SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title_full_unstemmed | SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title_short | SNORA71A Promotes Colorectal Cancer Cell Proliferation, Migration, and Invasion |
title_sort | snora71a promotes colorectal cancer cell proliferation, migration, and invasion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559222/ https://www.ncbi.nlm.nih.gov/pubmed/33083486 http://dx.doi.org/10.1155/2020/8284576 |
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