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Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II

African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsor...

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Autores principales: Rodríguez-Bertos, Antonio, Cadenas-Fernández, Estefanía, Rebollada-Merino, Agustín, Porras-González, Néstor, Mayoral-Alegre, Francisco J., Barreno, Lucía, Kosowska, Aleksandra, Tomé-Sánchez, Irene, Barasona, José A., Sánchez-Vizcaíno, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559345/
https://www.ncbi.nlm.nih.gov/pubmed/32842614
http://dx.doi.org/10.3390/pathogens9090688
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author Rodríguez-Bertos, Antonio
Cadenas-Fernández, Estefanía
Rebollada-Merino, Agustín
Porras-González, Néstor
Mayoral-Alegre, Francisco J.
Barreno, Lucía
Kosowska, Aleksandra
Tomé-Sánchez, Irene
Barasona, José A.
Sánchez-Vizcaíno, José M.
author_facet Rodríguez-Bertos, Antonio
Cadenas-Fernández, Estefanía
Rebollada-Merino, Agustín
Porras-González, Néstor
Mayoral-Alegre, Francisco J.
Barreno, Lucía
Kosowska, Aleksandra
Tomé-Sánchez, Irene
Barasona, José A.
Sánchez-Vizcaíno, José M.
author_sort Rodríguez-Bertos, Antonio
collection PubMed
description African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsorbing genotype II ASF virus (ASFV) strain. To this end, six wild boars were intramuscularly inoculated with the 10 HAD50 Arm07 ASFV strain, and 11 wild boars were allowed to come into direct contact with the inoculated animals. No animals survived the infection. Clinical course, gross pathological findings and viral genome quantification by PCR in tissues did not differ between intramuscularly inoculated or contact-infected animals. Postmortem analysis showed enlargement of liver and spleen; serosanguinous effusion in body cavities; and multiple hemorrhages in lungs, endocardium, brain, kidneys, urinary bladder, pancreas, and alimentary system. These results provide detailed insights into the gross pathology of wild boar infected with a highly virulent genotype II ASFV strain. From a didactic point of view, this detailed clinical course and macroscopic description may be essential for early postmortem detection of outbreaks in wild boar in the field and contribute to disease surveillance and prevention efforts.
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spelling pubmed-75593452020-10-29 Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II Rodríguez-Bertos, Antonio Cadenas-Fernández, Estefanía Rebollada-Merino, Agustín Porras-González, Néstor Mayoral-Alegre, Francisco J. Barreno, Lucía Kosowska, Aleksandra Tomé-Sánchez, Irene Barasona, José A. Sánchez-Vizcaíno, José M. Pathogens Article African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsorbing genotype II ASF virus (ASFV) strain. To this end, six wild boars were intramuscularly inoculated with the 10 HAD50 Arm07 ASFV strain, and 11 wild boars were allowed to come into direct contact with the inoculated animals. No animals survived the infection. Clinical course, gross pathological findings and viral genome quantification by PCR in tissues did not differ between intramuscularly inoculated or contact-infected animals. Postmortem analysis showed enlargement of liver and spleen; serosanguinous effusion in body cavities; and multiple hemorrhages in lungs, endocardium, brain, kidneys, urinary bladder, pancreas, and alimentary system. These results provide detailed insights into the gross pathology of wild boar infected with a highly virulent genotype II ASFV strain. From a didactic point of view, this detailed clinical course and macroscopic description may be essential for early postmortem detection of outbreaks in wild boar in the field and contribute to disease surveillance and prevention efforts. MDPI 2020-08-22 /pmc/articles/PMC7559345/ /pubmed/32842614 http://dx.doi.org/10.3390/pathogens9090688 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Bertos, Antonio
Cadenas-Fernández, Estefanía
Rebollada-Merino, Agustín
Porras-González, Néstor
Mayoral-Alegre, Francisco J.
Barreno, Lucía
Kosowska, Aleksandra
Tomé-Sánchez, Irene
Barasona, José A.
Sánchez-Vizcaíno, José M.
Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title_full Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title_fullStr Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title_full_unstemmed Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title_short Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
title_sort clinical course and gross pathological findings in wild boar infected with a highly virulent strain of african swine fever virus genotype ii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559345/
https://www.ncbi.nlm.nih.gov/pubmed/32842614
http://dx.doi.org/10.3390/pathogens9090688
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