Cargando…
Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II
African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsor...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559345/ https://www.ncbi.nlm.nih.gov/pubmed/32842614 http://dx.doi.org/10.3390/pathogens9090688 |
_version_ | 1783594840108302336 |
---|---|
author | Rodríguez-Bertos, Antonio Cadenas-Fernández, Estefanía Rebollada-Merino, Agustín Porras-González, Néstor Mayoral-Alegre, Francisco J. Barreno, Lucía Kosowska, Aleksandra Tomé-Sánchez, Irene Barasona, José A. Sánchez-Vizcaíno, José M. |
author_facet | Rodríguez-Bertos, Antonio Cadenas-Fernández, Estefanía Rebollada-Merino, Agustín Porras-González, Néstor Mayoral-Alegre, Francisco J. Barreno, Lucía Kosowska, Aleksandra Tomé-Sánchez, Irene Barasona, José A. Sánchez-Vizcaíno, José M. |
author_sort | Rodríguez-Bertos, Antonio |
collection | PubMed |
description | African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsorbing genotype II ASF virus (ASFV) strain. To this end, six wild boars were intramuscularly inoculated with the 10 HAD50 Arm07 ASFV strain, and 11 wild boars were allowed to come into direct contact with the inoculated animals. No animals survived the infection. Clinical course, gross pathological findings and viral genome quantification by PCR in tissues did not differ between intramuscularly inoculated or contact-infected animals. Postmortem analysis showed enlargement of liver and spleen; serosanguinous effusion in body cavities; and multiple hemorrhages in lungs, endocardium, brain, kidneys, urinary bladder, pancreas, and alimentary system. These results provide detailed insights into the gross pathology of wild boar infected with a highly virulent genotype II ASFV strain. From a didactic point of view, this detailed clinical course and macroscopic description may be essential for early postmortem detection of outbreaks in wild boar in the field and contribute to disease surveillance and prevention efforts. |
format | Online Article Text |
id | pubmed-7559345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75593452020-10-29 Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II Rodríguez-Bertos, Antonio Cadenas-Fernández, Estefanía Rebollada-Merino, Agustín Porras-González, Néstor Mayoral-Alegre, Francisco J. Barreno, Lucía Kosowska, Aleksandra Tomé-Sánchez, Irene Barasona, José A. Sánchez-Vizcaíno, José M. Pathogens Article African swine fever (ASF) is a notifiable disease that in recent years has spread remarkably in Europe and Asia. Eurasian wild boar (Sus scrofa) plays a key role in the maintenance and spread of the pathogen. Here we examined gross pathology of infection in wild boar with a highly virulent, hemadsorbing genotype II ASF virus (ASFV) strain. To this end, six wild boars were intramuscularly inoculated with the 10 HAD50 Arm07 ASFV strain, and 11 wild boars were allowed to come into direct contact with the inoculated animals. No animals survived the infection. Clinical course, gross pathological findings and viral genome quantification by PCR in tissues did not differ between intramuscularly inoculated or contact-infected animals. Postmortem analysis showed enlargement of liver and spleen; serosanguinous effusion in body cavities; and multiple hemorrhages in lungs, endocardium, brain, kidneys, urinary bladder, pancreas, and alimentary system. These results provide detailed insights into the gross pathology of wild boar infected with a highly virulent genotype II ASFV strain. From a didactic point of view, this detailed clinical course and macroscopic description may be essential for early postmortem detection of outbreaks in wild boar in the field and contribute to disease surveillance and prevention efforts. MDPI 2020-08-22 /pmc/articles/PMC7559345/ /pubmed/32842614 http://dx.doi.org/10.3390/pathogens9090688 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rodríguez-Bertos, Antonio Cadenas-Fernández, Estefanía Rebollada-Merino, Agustín Porras-González, Néstor Mayoral-Alegre, Francisco J. Barreno, Lucía Kosowska, Aleksandra Tomé-Sánchez, Irene Barasona, José A. Sánchez-Vizcaíno, José M. Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title | Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title_full | Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title_fullStr | Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title_full_unstemmed | Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title_short | Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II |
title_sort | clinical course and gross pathological findings in wild boar infected with a highly virulent strain of african swine fever virus genotype ii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559345/ https://www.ncbi.nlm.nih.gov/pubmed/32842614 http://dx.doi.org/10.3390/pathogens9090688 |
work_keys_str_mv | AT rodriguezbertosantonio clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT cadenasfernandezestefania clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT rebolladamerinoagustin clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT porrasgonzaleznestor clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT mayoralalegrefranciscoj clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT barrenolucia clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT kosowskaaleksandra clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT tomesanchezirene clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT barasonajosea clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii AT sanchezvizcainojosem clinicalcourseandgrosspathologicalfindingsinwildboarinfectedwithahighlyvirulentstrainofafricanswinefevervirusgenotypeii |