Cargando…
Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes
The main organochlorinated compounds used on agricultural crops are often recalcitrant, affecting nontarget organisms and contaminating rivers or groundwater. Diuron (N-(3,4-dichlorophenyl)-N′,N′-dimethylurea) is a chlorinated herbicide widely used in sugarcane plantations. Here, we evaluated the ab...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559502/ https://www.ncbi.nlm.nih.gov/pubmed/33083471 http://dx.doi.org/10.1155/2020/5324391 |
_version_ | 1783594875417001984 |
---|---|
author | Henn, Caroline Arakaki, Ricardo M. Monteiro, Diego Alves Boscolo, Mauricio da Silva, Roberto Gomes, Eleni |
author_facet | Henn, Caroline Arakaki, Ricardo M. Monteiro, Diego Alves Boscolo, Mauricio da Silva, Roberto Gomes, Eleni |
author_sort | Henn, Caroline |
collection | PubMed |
description | The main organochlorinated compounds used on agricultural crops are often recalcitrant, affecting nontarget organisms and contaminating rivers or groundwater. Diuron (N-(3,4-dichlorophenyl)-N′,N′-dimethylurea) is a chlorinated herbicide widely used in sugarcane plantations. Here, we evaluated the ability of 13 basidiomycete strains of growing in a contaminated culture medium and degrading the xenobiotic. Dissipation rates in culture medium with initial 25 mg/L of diuron ranged from 7.3 to 96.8%, being Pluteus cubensis SXS 320 the most efficient strain, leaving no detectable residues after diuron metabolism. Pycnoporus sanguineus MCA 16 removed 56% of diuron after 40 days of cultivation, producing three metabolites more polar than parental herbicide, two of them identified as being DCPU and DCPMU. Despite of the strong inductive effect of diuron upon laccase synthesis and secretion, the application of crude enzymatic extracts of P. sanguineus did not catalyzed the breakdown of the herbicide in vitro, indicating that diuron biodegradation was not related to this oxidative enzyme. |
format | Online Article Text |
id | pubmed-7559502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75595022020-10-19 Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes Henn, Caroline Arakaki, Ricardo M. Monteiro, Diego Alves Boscolo, Mauricio da Silva, Roberto Gomes, Eleni Biomed Res Int Research Article The main organochlorinated compounds used on agricultural crops are often recalcitrant, affecting nontarget organisms and contaminating rivers or groundwater. Diuron (N-(3,4-dichlorophenyl)-N′,N′-dimethylurea) is a chlorinated herbicide widely used in sugarcane plantations. Here, we evaluated the ability of 13 basidiomycete strains of growing in a contaminated culture medium and degrading the xenobiotic. Dissipation rates in culture medium with initial 25 mg/L of diuron ranged from 7.3 to 96.8%, being Pluteus cubensis SXS 320 the most efficient strain, leaving no detectable residues after diuron metabolism. Pycnoporus sanguineus MCA 16 removed 56% of diuron after 40 days of cultivation, producing three metabolites more polar than parental herbicide, two of them identified as being DCPU and DCPMU. Despite of the strong inductive effect of diuron upon laccase synthesis and secretion, the application of crude enzymatic extracts of P. sanguineus did not catalyzed the breakdown of the herbicide in vitro, indicating that diuron biodegradation was not related to this oxidative enzyme. Hindawi 2020-10-06 /pmc/articles/PMC7559502/ /pubmed/33083471 http://dx.doi.org/10.1155/2020/5324391 Text en Copyright © 2020 Caroline Henn et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Henn, Caroline Arakaki, Ricardo M. Monteiro, Diego Alves Boscolo, Mauricio da Silva, Roberto Gomes, Eleni Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title | Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title_full | Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title_fullStr | Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title_full_unstemmed | Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title_short | Degradation of the Organochlorinated Herbicide Diuron by Rainforest Basidiomycetes |
title_sort | degradation of the organochlorinated herbicide diuron by rainforest basidiomycetes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559502/ https://www.ncbi.nlm.nih.gov/pubmed/33083471 http://dx.doi.org/10.1155/2020/5324391 |
work_keys_str_mv | AT henncaroline degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes AT arakakiricardom degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes AT monteirodiegoalves degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes AT boscolomauricio degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes AT dasilvaroberto degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes AT gomeseleni degradationoftheorganochlorinatedherbicidediuronbyrainforestbasidiomycetes |