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Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

The growing interest in the development of green pest management strategies is leading to the exploitation of essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support the use of EOs through their encapsulation into stable nanoformulations, suc...

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Autores principales: Benelli, Giovanni, Pavoni, Lucia, Zeni, Valeria, Ricciardi, Renato, Cosci, Francesca, Cacopardo, Gloria, Gendusa, Saverio, Spinozzi, Eleonora, Petrelli, Riccardo, Cappellacci, Loredana, Maggi, Filippo, Pavela, Roman, Bonacucina, Giulia, Lucchi, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559805/
https://www.ncbi.nlm.nih.gov/pubmed/32961890
http://dx.doi.org/10.3390/nano10091867
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author Benelli, Giovanni
Pavoni, Lucia
Zeni, Valeria
Ricciardi, Renato
Cosci, Francesca
Cacopardo, Gloria
Gendusa, Saverio
Spinozzi, Eleonora
Petrelli, Riccardo
Cappellacci, Loredana
Maggi, Filippo
Pavela, Roman
Bonacucina, Giulia
Lucchi, Andrea
author_facet Benelli, Giovanni
Pavoni, Lucia
Zeni, Valeria
Ricciardi, Renato
Cosci, Francesca
Cacopardo, Gloria
Gendusa, Saverio
Spinozzi, Eleonora
Petrelli, Riccardo
Cappellacci, Loredana
Maggi, Filippo
Pavela, Roman
Bonacucina, Giulia
Lucchi, Andrea
author_sort Benelli, Giovanni
collection PubMed
description The growing interest in the development of green pest management strategies is leading to the exploitation of essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support the use of EOs through their encapsulation into stable nanoformulations, such as nanoemulsions (NEs), to improve their stability and efficacy. This technology assures the improvement of the chemical stability, hydrophilicity, and environmental persistence of EOs, giving an added value for the fabrication of natural insecticides effective against a wide spectrum of insect vectors and pests of public and agronomical importance. Carlina acaulis (Asteraceae) root EO has been recently proposed as a promising ingredient of a new generation of botanical insecticides. In the present study, a highly stable C. acaulis-based NE was developed. Interestingly, such a nanosystem was able to encapsulate 6% (w/w) of C. acaulis EO, showing a mean diameter of around 140 nm and a SOR (surfactant-to-oil ratio) of 0.6. Its stability was evaluated in a storage period of six months and corroborated by an accelerated stability study. Therefore, the C. acaulis EO and C. acaulis-based NE were evaluated for their toxicity against 1st instar larvae of the European grapevine moth (EGVM), Lobesia botrana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae), a major vineyard pest. The chemical composition of C. acaulis EO was investigated by gas chromatography–mass spectrometry (GC–MS) revealing carlina oxide, a polyacetylene, as the main constituent. In toxicity assays, both the C. acaulis EO and the C. acaulis-based NE were highly toxic to L. botrana larvae, with LC(50) values of 7.299 and 9.044 µL/mL for C. acaulis EO and NE, respectively. The C. acaulis-based NE represents a promising option to develop highly stable botanical insecticides for pest management. To date, this study represents the first evidence about the insecticidal toxicity of EOs and EO-based NEs against this major grapevine pest.
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spelling pubmed-75598052020-10-29 Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana Benelli, Giovanni Pavoni, Lucia Zeni, Valeria Ricciardi, Renato Cosci, Francesca Cacopardo, Gloria Gendusa, Saverio Spinozzi, Eleonora Petrelli, Riccardo Cappellacci, Loredana Maggi, Filippo Pavela, Roman Bonacucina, Giulia Lucchi, Andrea Nanomaterials (Basel) Article The growing interest in the development of green pest management strategies is leading to the exploitation of essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support the use of EOs through their encapsulation into stable nanoformulations, such as nanoemulsions (NEs), to improve their stability and efficacy. This technology assures the improvement of the chemical stability, hydrophilicity, and environmental persistence of EOs, giving an added value for the fabrication of natural insecticides effective against a wide spectrum of insect vectors and pests of public and agronomical importance. Carlina acaulis (Asteraceae) root EO has been recently proposed as a promising ingredient of a new generation of botanical insecticides. In the present study, a highly stable C. acaulis-based NE was developed. Interestingly, such a nanosystem was able to encapsulate 6% (w/w) of C. acaulis EO, showing a mean diameter of around 140 nm and a SOR (surfactant-to-oil ratio) of 0.6. Its stability was evaluated in a storage period of six months and corroborated by an accelerated stability study. Therefore, the C. acaulis EO and C. acaulis-based NE were evaluated for their toxicity against 1st instar larvae of the European grapevine moth (EGVM), Lobesia botrana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae), a major vineyard pest. The chemical composition of C. acaulis EO was investigated by gas chromatography–mass spectrometry (GC–MS) revealing carlina oxide, a polyacetylene, as the main constituent. In toxicity assays, both the C. acaulis EO and the C. acaulis-based NE were highly toxic to L. botrana larvae, with LC(50) values of 7.299 and 9.044 µL/mL for C. acaulis EO and NE, respectively. The C. acaulis-based NE represents a promising option to develop highly stable botanical insecticides for pest management. To date, this study represents the first evidence about the insecticidal toxicity of EOs and EO-based NEs against this major grapevine pest. MDPI 2020-09-18 /pmc/articles/PMC7559805/ /pubmed/32961890 http://dx.doi.org/10.3390/nano10091867 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benelli, Giovanni
Pavoni, Lucia
Zeni, Valeria
Ricciardi, Renato
Cosci, Francesca
Cacopardo, Gloria
Gendusa, Saverio
Spinozzi, Eleonora
Petrelli, Riccardo
Cappellacci, Loredana
Maggi, Filippo
Pavela, Roman
Bonacucina, Giulia
Lucchi, Andrea
Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title_full Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title_fullStr Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title_full_unstemmed Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title_short Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana
title_sort developing a highly stable carlina acaulis essential oil nanoemulsion for managing lobesia botrana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559805/
https://www.ncbi.nlm.nih.gov/pubmed/32961890
http://dx.doi.org/10.3390/nano10091867
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