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Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay

The incidence of enteric viruses in treated wastewater and their potential release into the environment or use for agriculture are very critical matters in public health. In our study, PCR (polymerase chain reaction) analysis of enteric viruses was performed on 59 samples of influents and effluents...

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Autores principales: Janahi, Essam M., Mustafa, Sakina, Parkar, Saba F. D., Naser, Humood A., Eisa, Zaki M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559856/
https://www.ncbi.nlm.nih.gov/pubmed/32899918
http://dx.doi.org/10.3390/ijerph17186483
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author Janahi, Essam M.
Mustafa, Sakina
Parkar, Saba F. D.
Naser, Humood A.
Eisa, Zaki M.
author_facet Janahi, Essam M.
Mustafa, Sakina
Parkar, Saba F. D.
Naser, Humood A.
Eisa, Zaki M.
author_sort Janahi, Essam M.
collection PubMed
description The incidence of enteric viruses in treated wastewater and their potential release into the environment or use for agriculture are very critical matters in public health. In our study, PCR (polymerase chain reaction) analysis of enteric viruses was performed on 59 samples of influents and effluents collected from Tubli wastewater treatment plant (Water Pollution Control Center (WPCC)) and Tubli Bay, where the effluents were discharged, in Kingdom of Bahrain during two sampling periods. Four clinically essential waterborne enteric viruses were examined: enterovirus (EV), hepatitis A virus (HAV), astroviruses (AV), and rotaviruses (RV) and compared to standard bacterial and bacteriophages indicators of fecal pollution. Detection rates of EV, AV, HAV, and RV in the influent samples were 100%, 75%, 12.5%, and 12.5%, respectively, while 50% of the effluent samples from Tubli WPCC contained only EV RNA. None of the tested enteric viruses could be detected in any of the samples collected directly from Tubli Bay. Effluent samples from Tubli plant did not show significant seasonal differences. Since detection of enteric viruses genome does not necessarily indicate infectivity, the infectivity of these viruses was evaluated through isolation and growth of indictor bacteria and bacteriophages. High concentration of fecal bacteriological indicators was detected in all effluents samples (100%): 3.20 × 10(3) cfu/mL for E. coli, 1.32 × 10(3) cfu/mL for Salmonella spp., and 1.92 × 10(3) cfu/mL for Shigella spp. E. coli and Salmonella specific bacteriophages were also detected in the effluent samples in high titers. The combined results of PCR and bacterial enumeration point to a probable public health risk via the use of these wastewaters in agriculture or their discharge into the sea. Continuous surveillance of viral and bacterial prevalence and their resistance to sewage disinfection procedures could contribute to a better control of risks associated with the recycling of effluent wastewater and its release into the environment.
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spelling pubmed-75598562020-10-22 Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay Janahi, Essam M. Mustafa, Sakina Parkar, Saba F. D. Naser, Humood A. Eisa, Zaki M. Int J Environ Res Public Health Article The incidence of enteric viruses in treated wastewater and their potential release into the environment or use for agriculture are very critical matters in public health. In our study, PCR (polymerase chain reaction) analysis of enteric viruses was performed on 59 samples of influents and effluents collected from Tubli wastewater treatment plant (Water Pollution Control Center (WPCC)) and Tubli Bay, where the effluents were discharged, in Kingdom of Bahrain during two sampling periods. Four clinically essential waterborne enteric viruses were examined: enterovirus (EV), hepatitis A virus (HAV), astroviruses (AV), and rotaviruses (RV) and compared to standard bacterial and bacteriophages indicators of fecal pollution. Detection rates of EV, AV, HAV, and RV in the influent samples were 100%, 75%, 12.5%, and 12.5%, respectively, while 50% of the effluent samples from Tubli WPCC contained only EV RNA. None of the tested enteric viruses could be detected in any of the samples collected directly from Tubli Bay. Effluent samples from Tubli plant did not show significant seasonal differences. Since detection of enteric viruses genome does not necessarily indicate infectivity, the infectivity of these viruses was evaluated through isolation and growth of indictor bacteria and bacteriophages. High concentration of fecal bacteriological indicators was detected in all effluents samples (100%): 3.20 × 10(3) cfu/mL for E. coli, 1.32 × 10(3) cfu/mL for Salmonella spp., and 1.92 × 10(3) cfu/mL for Shigella spp. E. coli and Salmonella specific bacteriophages were also detected in the effluent samples in high titers. The combined results of PCR and bacterial enumeration point to a probable public health risk via the use of these wastewaters in agriculture or their discharge into the sea. Continuous surveillance of viral and bacterial prevalence and their resistance to sewage disinfection procedures could contribute to a better control of risks associated with the recycling of effluent wastewater and its release into the environment. MDPI 2020-09-06 2020-09 /pmc/articles/PMC7559856/ /pubmed/32899918 http://dx.doi.org/10.3390/ijerph17186483 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Janahi, Essam M.
Mustafa, Sakina
Parkar, Saba F. D.
Naser, Humood A.
Eisa, Zaki M.
Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title_full Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title_fullStr Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title_full_unstemmed Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title_short Detection of Enteric Viruses and Bacterial Indicators in a Sewage Treatment Center and Shallow Water Bay
title_sort detection of enteric viruses and bacterial indicators in a sewage treatment center and shallow water bay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559856/
https://www.ncbi.nlm.nih.gov/pubmed/32899918
http://dx.doi.org/10.3390/ijerph17186483
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