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Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission

Mosquitoes are the most important vectors for arthropod-borne viral diseases. Mixed viral infections of mosquitoes allow genetic recombination or reassortment of diverse viruses, turning mosquitoes into potential virologic mixing bowls. In this study, we field-collected mosquitoes of different speci...

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Autores principales: Thannesberger, Jakob, Rascovan, Nicolas, Eisenmann, Anna, Klymiuk, Ingeborg, Zittra, Carina, Fuehrer, Hans-Peter, Scantlebury-Manning, Thea, Gittens-St.Hilaire, Marquita, Austin, Shane, Landis, Robert Clive, Steininger, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559857/
https://www.ncbi.nlm.nih.gov/pubmed/32839419
http://dx.doi.org/10.3390/pathogens9090686
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author Thannesberger, Jakob
Rascovan, Nicolas
Eisenmann, Anna
Klymiuk, Ingeborg
Zittra, Carina
Fuehrer, Hans-Peter
Scantlebury-Manning, Thea
Gittens-St.Hilaire, Marquita
Austin, Shane
Landis, Robert Clive
Steininger, Christoph
author_facet Thannesberger, Jakob
Rascovan, Nicolas
Eisenmann, Anna
Klymiuk, Ingeborg
Zittra, Carina
Fuehrer, Hans-Peter
Scantlebury-Manning, Thea
Gittens-St.Hilaire, Marquita
Austin, Shane
Landis, Robert Clive
Steininger, Christoph
author_sort Thannesberger, Jakob
collection PubMed
description Mosquitoes are the most important vectors for arthropod-borne viral diseases. Mixed viral infections of mosquitoes allow genetic recombination or reassortment of diverse viruses, turning mosquitoes into potential virologic mixing bowls. In this study, we field-collected mosquitoes of different species (Aedes aegypti and Culex pipiens complex), from different geographic locations and environments (central Europe and the Caribbean) for highly sensitive next-generation sequencing-based virome characterization. We found a rich virus community associated with a great diversity of host species. Among those, we detected a large diversity of novel virus sequences that we could predominately assign to circular Rep-encoding single-stranded (CRESS) DNA viruses, including the full-length genome of a yet undescribed Gemykrogvirus species. Moreover, we report for the first time the detection of a potentially zoonotic CRESS-DNA virus (Cyclovirus VN) in mosquito vectors. This study expands the knowledge on virus diversity in medically important mosquito vectors, especially for CRESS-DNA viruses that have previously been shown to easily recombine and jump the species barrier.
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spelling pubmed-75598572020-10-22 Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission Thannesberger, Jakob Rascovan, Nicolas Eisenmann, Anna Klymiuk, Ingeborg Zittra, Carina Fuehrer, Hans-Peter Scantlebury-Manning, Thea Gittens-St.Hilaire, Marquita Austin, Shane Landis, Robert Clive Steininger, Christoph Pathogens Article Mosquitoes are the most important vectors for arthropod-borne viral diseases. Mixed viral infections of mosquitoes allow genetic recombination or reassortment of diverse viruses, turning mosquitoes into potential virologic mixing bowls. In this study, we field-collected mosquitoes of different species (Aedes aegypti and Culex pipiens complex), from different geographic locations and environments (central Europe and the Caribbean) for highly sensitive next-generation sequencing-based virome characterization. We found a rich virus community associated with a great diversity of host species. Among those, we detected a large diversity of novel virus sequences that we could predominately assign to circular Rep-encoding single-stranded (CRESS) DNA viruses, including the full-length genome of a yet undescribed Gemykrogvirus species. Moreover, we report for the first time the detection of a potentially zoonotic CRESS-DNA virus (Cyclovirus VN) in mosquito vectors. This study expands the knowledge on virus diversity in medically important mosquito vectors, especially for CRESS-DNA viruses that have previously been shown to easily recombine and jump the species barrier. MDPI 2020-08-21 /pmc/articles/PMC7559857/ /pubmed/32839419 http://dx.doi.org/10.3390/pathogens9090686 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thannesberger, Jakob
Rascovan, Nicolas
Eisenmann, Anna
Klymiuk, Ingeborg
Zittra, Carina
Fuehrer, Hans-Peter
Scantlebury-Manning, Thea
Gittens-St.Hilaire, Marquita
Austin, Shane
Landis, Robert Clive
Steininger, Christoph
Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title_full Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title_fullStr Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title_full_unstemmed Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title_short Highly Sensitive Virome Characterization of Aedes aegypti and Culex pipiens Complex from Central Europe and the Caribbean Reveals Potential for Interspecies Viral Transmission
title_sort highly sensitive virome characterization of aedes aegypti and culex pipiens complex from central europe and the caribbean reveals potential for interspecies viral transmission
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559857/
https://www.ncbi.nlm.nih.gov/pubmed/32839419
http://dx.doi.org/10.3390/pathogens9090686
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