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Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection

Interkingdom polymicrobial diseases are caused by different microorganisms that colonize the same niche, as in the case of yeast-bacteria coinfections. The latter are difficult to treat due the absence of any common therapeutic target for their elimination, both in animals and humans. Staphylococcus...

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Autores principales: Bellavita, Rosa, Vollaro, Adriana, Catania, Maria Rosaria, Merlino, Francesco, De Martino, Luisa, Nocera, Francesca Paola, DellaGreca, Marina, Lembo, Francesca, Grieco, Paolo, Buommino, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560154/
https://www.ncbi.nlm.nih.gov/pubmed/32842593
http://dx.doi.org/10.3390/antibiotics9090530
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author Bellavita, Rosa
Vollaro, Adriana
Catania, Maria Rosaria
Merlino, Francesco
De Martino, Luisa
Nocera, Francesca Paola
DellaGreca, Marina
Lembo, Francesca
Grieco, Paolo
Buommino, Elisabetta
author_facet Bellavita, Rosa
Vollaro, Adriana
Catania, Maria Rosaria
Merlino, Francesco
De Martino, Luisa
Nocera, Francesca Paola
DellaGreca, Marina
Lembo, Francesca
Grieco, Paolo
Buommino, Elisabetta
author_sort Bellavita, Rosa
collection PubMed
description Interkingdom polymicrobial diseases are caused by different microorganisms that colonize the same niche, as in the case of yeast-bacteria coinfections. The latter are difficult to treat due the absence of any common therapeutic target for their elimination, both in animals and humans. Staphylococcus pseudintermedius and Malassezia pachydermatis belong to distinct kingdoms. They can colonize the same skin district or apparatus being the causative agents of fastidious pet animals’ pathologies. Here we analysed the antimicrobial properties of a panel of 11 peptides, derived from temporin L, against Malassezia pachydermatis. Only peptide 8 showed the best mycocidal activity at 6.25 μM. Prolonged application of peptide 8 did not cause M. pachydermatis drug-resistance. Peptide 8 was also able to inhibit the growth of Staphylococcus pseudintermedius, regardless of methicillin resistance, at 1.56 μM for methicillin-susceptible S. pseudintermedius (MSSP) and 6.25 μM for methicillin-resistant S. pseudintermedius (MRSP). Of interest, peptide 8 increased the susceptibility of MRSP to oxacillin. Oxacillin MIC value reduction was of about eight times when used in combination with peptide 8. Finally, the compound affected the vitality of bacteria embedded in S. pseudintermedius biofilm. In conclusion, peptide 8 might represent a valid therapeutic alternative in the treatment of interkingdom polymicrobial infections, also in the presence of methicillin-resistant bacteria.
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spelling pubmed-75601542020-10-22 Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection Bellavita, Rosa Vollaro, Adriana Catania, Maria Rosaria Merlino, Francesco De Martino, Luisa Nocera, Francesca Paola DellaGreca, Marina Lembo, Francesca Grieco, Paolo Buommino, Elisabetta Antibiotics (Basel) Article Interkingdom polymicrobial diseases are caused by different microorganisms that colonize the same niche, as in the case of yeast-bacteria coinfections. The latter are difficult to treat due the absence of any common therapeutic target for their elimination, both in animals and humans. Staphylococcus pseudintermedius and Malassezia pachydermatis belong to distinct kingdoms. They can colonize the same skin district or apparatus being the causative agents of fastidious pet animals’ pathologies. Here we analysed the antimicrobial properties of a panel of 11 peptides, derived from temporin L, against Malassezia pachydermatis. Only peptide 8 showed the best mycocidal activity at 6.25 μM. Prolonged application of peptide 8 did not cause M. pachydermatis drug-resistance. Peptide 8 was also able to inhibit the growth of Staphylococcus pseudintermedius, regardless of methicillin resistance, at 1.56 μM for methicillin-susceptible S. pseudintermedius (MSSP) and 6.25 μM for methicillin-resistant S. pseudintermedius (MRSP). Of interest, peptide 8 increased the susceptibility of MRSP to oxacillin. Oxacillin MIC value reduction was of about eight times when used in combination with peptide 8. Finally, the compound affected the vitality of bacteria embedded in S. pseudintermedius biofilm. In conclusion, peptide 8 might represent a valid therapeutic alternative in the treatment of interkingdom polymicrobial infections, also in the presence of methicillin-resistant bacteria. MDPI 2020-08-22 /pmc/articles/PMC7560154/ /pubmed/32842593 http://dx.doi.org/10.3390/antibiotics9090530 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bellavita, Rosa
Vollaro, Adriana
Catania, Maria Rosaria
Merlino, Francesco
De Martino, Luisa
Nocera, Francesca Paola
DellaGreca, Marina
Lembo, Francesca
Grieco, Paolo
Buommino, Elisabetta
Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title_full Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title_fullStr Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title_full_unstemmed Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title_short Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection
title_sort novel antimicrobial peptide from temporin l in the treatment of staphylococcus pseudintermedius and malassezia pachydermatis in polymicrobial inter-kingdom infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560154/
https://www.ncbi.nlm.nih.gov/pubmed/32842593
http://dx.doi.org/10.3390/antibiotics9090530
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