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Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions

The use of new agricultural technologies such as soilless and aeroponic cultivation systems is a valuable approach to medicinal plant production. The present study investigated the prospects of enhancing yield and secondary metabolite production in Valeriana jatamansi under aeroponic cultivation usi...

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Autores principales: Partap, Mahinder, Kumar, Pankaj, Kumar, Anil, Joshi, Robin, Kumar, Dinesh, Warghat, Ashish R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561395/
https://www.ncbi.nlm.nih.gov/pubmed/33117410
http://dx.doi.org/10.3389/fpls.2020.01263
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author Partap, Mahinder
Kumar, Pankaj
Kumar, Anil
Joshi, Robin
Kumar, Dinesh
Warghat, Ashish R.
author_facet Partap, Mahinder
Kumar, Pankaj
Kumar, Anil
Joshi, Robin
Kumar, Dinesh
Warghat, Ashish R.
author_sort Partap, Mahinder
collection PubMed
description The use of new agricultural technologies such as soilless and aeroponic cultivation systems is a valuable approach to medicinal plant production. The present study investigated the prospects of enhancing yield and secondary metabolite production in Valeriana jatamansi under aeroponic cultivation using elicitors, such as yeast extract and methyl jasmonate. Plants were evaluated by measuring growth parameters, photosynthetic rate, and secondary metabolites contents (on a dry weight basis). Maximum plant height (36.83 cm), leaf number (17.67), rootlet number (37.33), and rootlet length (6.90 cm) were observed at 0.5 mg/L yeast extract treatment; whereas treatment levels of 1.5 mg/L yeast extract and 150 µM methyl jasmonate resulted in maximum leaf length (6.95 cm) and leaf width (5.43 cm), respectively. Maximum photosynthetic rate (5.4053 µmol m(-2)s(-1)) and stomatal conductance (0.0656 mmol m(-2)s(-1)) were recorded at treatment levels of 0.5 mg/L and 1.5 mg/L yeast extract respectively, whereas at 150 µM methyl jasmonate treatment, transpiration rate was 0.9046 mmol m(-2)s(-1). In aeroponic cultivation, the maximum content of valerenic acid and hydroxy valerenic acid was detected in leaf (2.47 and 8.37 mg/g) and root (1.78 and 7.89 mg/g) at treatment levels of 100 µM and 150 µM methyl jasmonate, respectively. Acetoxy valerenic acid was highest in leaf (1.02 mg/g) at 1.5 mg/L yeast extract, and in the root (2.38 mg/g) at 150 µM methyl jasmonate. Gas chromatography-mass spectrometry analysis identified twenty-eight volatile compounds in roots, of which three—isovaleric acid (6.72-50.81%), patchouli alcohol (13.48-25.31%) and baldrinal (0.74-25.26%)—were the major constituents. The results revealed that, besides roots, leaves could also be utilized as a prominent alternative source for targeted secondary metabolites. In conclusion, aeroponic cultivation offers year-round quality biomass production and ease to access subsequent roots harvest in V. jatamansi, to meet the demand of the pharmaceutical industries.
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spelling pubmed-75613952020-10-27 Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions Partap, Mahinder Kumar, Pankaj Kumar, Anil Joshi, Robin Kumar, Dinesh Warghat, Ashish R. Front Plant Sci Plant Science The use of new agricultural technologies such as soilless and aeroponic cultivation systems is a valuable approach to medicinal plant production. The present study investigated the prospects of enhancing yield and secondary metabolite production in Valeriana jatamansi under aeroponic cultivation using elicitors, such as yeast extract and methyl jasmonate. Plants were evaluated by measuring growth parameters, photosynthetic rate, and secondary metabolites contents (on a dry weight basis). Maximum plant height (36.83 cm), leaf number (17.67), rootlet number (37.33), and rootlet length (6.90 cm) were observed at 0.5 mg/L yeast extract treatment; whereas treatment levels of 1.5 mg/L yeast extract and 150 µM methyl jasmonate resulted in maximum leaf length (6.95 cm) and leaf width (5.43 cm), respectively. Maximum photosynthetic rate (5.4053 µmol m(-2)s(-1)) and stomatal conductance (0.0656 mmol m(-2)s(-1)) were recorded at treatment levels of 0.5 mg/L and 1.5 mg/L yeast extract respectively, whereas at 150 µM methyl jasmonate treatment, transpiration rate was 0.9046 mmol m(-2)s(-1). In aeroponic cultivation, the maximum content of valerenic acid and hydroxy valerenic acid was detected in leaf (2.47 and 8.37 mg/g) and root (1.78 and 7.89 mg/g) at treatment levels of 100 µM and 150 µM methyl jasmonate, respectively. Acetoxy valerenic acid was highest in leaf (1.02 mg/g) at 1.5 mg/L yeast extract, and in the root (2.38 mg/g) at 150 µM methyl jasmonate. Gas chromatography-mass spectrometry analysis identified twenty-eight volatile compounds in roots, of which three—isovaleric acid (6.72-50.81%), patchouli alcohol (13.48-25.31%) and baldrinal (0.74-25.26%)—were the major constituents. The results revealed that, besides roots, leaves could also be utilized as a prominent alternative source for targeted secondary metabolites. In conclusion, aeroponic cultivation offers year-round quality biomass production and ease to access subsequent roots harvest in V. jatamansi, to meet the demand of the pharmaceutical industries. Frontiers Media S.A. 2020-09-30 /pmc/articles/PMC7561395/ /pubmed/33117410 http://dx.doi.org/10.3389/fpls.2020.01263 Text en Copyright © 2020 Partap, Kumar, Kumar, Joshi, Kumar and Warghat http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Partap, Mahinder
Kumar, Pankaj
Kumar, Anil
Joshi, Robin
Kumar, Dinesh
Warghat, Ashish R.
Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title_full Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title_fullStr Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title_full_unstemmed Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title_short Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions
title_sort effect of elicitors on morpho-physiological performance and metabolites enrichment in valeriana jatamansi cultivated under aeroponic conditions
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561395/
https://www.ncbi.nlm.nih.gov/pubmed/33117410
http://dx.doi.org/10.3389/fpls.2020.01263
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