Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols

This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individ...

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Autores principales: Volpato, Fabiana, Lima-Morales, Daiana, Wink, Priscila Lamb, Willig, Julia, de-Paris, Fernanda, Ashton-Prolla, Patricia, Barth, Afonso Luís
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561432/
https://www.ncbi.nlm.nih.gov/pubmed/33063233
http://dx.doi.org/10.1007/s10096-020-04071-8
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author Volpato, Fabiana
Lima-Morales, Daiana
Wink, Priscila Lamb
Willig, Julia
de-Paris, Fernanda
Ashton-Prolla, Patricia
Barth, Afonso Luís
author_facet Volpato, Fabiana
Lima-Morales, Daiana
Wink, Priscila Lamb
Willig, Julia
de-Paris, Fernanda
Ashton-Prolla, Patricia
Barth, Afonso Luís
author_sort Volpato, Fabiana
collection PubMed
description This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.
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spelling pubmed-75614322020-10-16 Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols Volpato, Fabiana Lima-Morales, Daiana Wink, Priscila Lamb Willig, Julia de-Paris, Fernanda Ashton-Prolla, Patricia Barth, Afonso Luís Eur J Clin Microbiol Infect Dis Brief Report This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2. Springer Berlin Heidelberg 2020-10-16 2021 /pmc/articles/PMC7561432/ /pubmed/33063233 http://dx.doi.org/10.1007/s10096-020-04071-8 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Volpato, Fabiana
Lima-Morales, Daiana
Wink, Priscila Lamb
Willig, Julia
de-Paris, Fernanda
Ashton-Prolla, Patricia
Barth, Afonso Luís
Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title_full Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title_fullStr Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title_full_unstemmed Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title_short Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
title_sort pooling of samples to optimize sars-cov-2 diagnosis by rt-qpcr: comparative analysis of two protocols
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561432/
https://www.ncbi.nlm.nih.gov/pubmed/33063233
http://dx.doi.org/10.1007/s10096-020-04071-8
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