Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen

Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Resu...

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Autores principales: Tomás, Ana Luísa, Cardoso, Fernando, de Sousa, Bruno, Matos, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561569/
https://www.ncbi.nlm.nih.gov/pubmed/32557324
http://dx.doi.org/10.1007/s10096-020-03936-2
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author Tomás, Ana Luísa
Cardoso, Fernando
de Sousa, Bruno
Matos, Olga
author_facet Tomás, Ana Luísa
Cardoso, Fernando
de Sousa, Bruno
Matos, Olga
author_sort Tomás, Ana Luísa
collection PubMed
description Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results showed that IgM anti-Kex1 levels were found significantly increased in patients with Pneumocystis pneumonia (PcP) compared with non-PcP cases (p < 0.001), allowing a diagnostic performance of PcP with a 70.8% sensitivity and a 75.0% specificity. These results suggest that this Kex1-based ELISA is a promising tool toward the serodiagnosis of PcP when the standard methods are difficult to perform.
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spelling pubmed-75615692020-10-19 Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen Tomás, Ana Luísa Cardoso, Fernando de Sousa, Bruno Matos, Olga Eur J Clin Microbiol Infect Dis Brief Report Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results showed that IgM anti-Kex1 levels were found significantly increased in patients with Pneumocystis pneumonia (PcP) compared with non-PcP cases (p < 0.001), allowing a diagnostic performance of PcP with a 70.8% sensitivity and a 75.0% specificity. These results suggest that this Kex1-based ELISA is a promising tool toward the serodiagnosis of PcP when the standard methods are difficult to perform. Springer Berlin Heidelberg 2020-06-16 2020 /pmc/articles/PMC7561569/ /pubmed/32557324 http://dx.doi.org/10.1007/s10096-020-03936-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Tomás, Ana Luísa
Cardoso, Fernando
de Sousa, Bruno
Matos, Olga
Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title_full Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title_fullStr Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title_full_unstemmed Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title_short Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
title_sort detection of anti-pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561569/
https://www.ncbi.nlm.nih.gov/pubmed/32557324
http://dx.doi.org/10.1007/s10096-020-03936-2
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