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Optimized DNA extraction and purification method for characterization of bacterial and fungal communities in lung tissue samples

Human lungs harbor a scarce microbial community, requiring to develop methods to enhance the recovery of nucleic acids from bacteria and fungi, leading to a more efficient analysis of the lung tissue microbiota. Here we describe five extraction protocols including pre-treatment, bead-beating and/or...

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Detalles Bibliográficos
Autores principales:	Pérez-Brocal, Vicente, Magne, Fabien, Ruiz-Ruiz, Susana, Ponce, Carolina A., Bustamante, Rebeca, Martin, Viviana San, Gutierrez, Mireya, Gatti, Gianna, Vargas, Sergio L., Moya, Andrés
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7562954/ https://www.ncbi.nlm.nih.gov/pubmed/33060634 http://dx.doi.org/10.1038/s41598-020-74137-2

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