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A comprehensive and contemporary “snapshot” of β-lactamases in carbapenem resistant Acinetobacter baumannii

Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii...

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Detalles Bibliográficos
Autores principales: Hujer, Andrea M., Hujer, Kristine M., Leonard, David A., Powers, Rachel A., Wallar, Bradley J., Mack, Andrew R., Taracila, Magdalena A., Rather, Philip N., Higgins, Paul G., Prati, Fabio, Caselli, Emilia, Marshall, Steven H., Clarke, Thomas, Greco, Christopher, Venepally, Pratap, Brinkac, Lauren, Kreiswirth, Barry N., Fouts, Derrick E., Bonomo, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7562987/
https://www.ncbi.nlm.nih.gov/pubmed/33248392
http://dx.doi.org/10.1016/j.diagmicrobio.2020.115242
Descripción
Sumario:Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired bla(OXA) carbapenemase was found in addition to the intrinsic bla(OXA) allele. The most commonly found allele was bla(OXA-23) (n = 78/98). In some isolates, bla(OXA-23) was found in addition to other carbapenemase alleles: bla(OXA-82) (n = 12/78), bla(OXA-72) (n = 2/78) and bla(OXA-24/40) (n = 1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., bla(OXA-82) and bla(OXA-172); all had ISAba1 elements. In 8 CR Ab, bla(OXA-82) or bla(OXA-172) was the only carbapenemase. Both bla(OXA-24/40) and its variant bla(OXA-72) were each found in 6/98 isolates. The most prevalent ADC variants were bla(ADC-30) (21%), bla(ADC-162) (21%), and bla(ADC-212) (26%). Complete combinations are reported.