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HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test

Background: High risk human papillomavirus (hr-HPV)-associated oropharyngeal cancers (OPCs) are characterized by significantly better therapy responses. In order to implement a de-escalated treatment strategy for this tumor entity, it is highly crucial to accurately distinguish HPV-associated OPCs f...

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Autores principales: Borena, Wegene, Schartinger, Volker H., Dudas, Jozsef, Ingruber, Julia, Greier, Maria C., Steinbichler, Teresa B., Laimer, Johannes, Stoiber, Heribert, Riechelmann, Herbert, Kofler, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563171/
https://www.ncbi.nlm.nih.gov/pubmed/32842554
http://dx.doi.org/10.3390/cancers12092388
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author Borena, Wegene
Schartinger, Volker H.
Dudas, Jozsef
Ingruber, Julia
Greier, Maria C.
Steinbichler, Teresa B.
Laimer, Johannes
Stoiber, Heribert
Riechelmann, Herbert
Kofler, Barbara
author_facet Borena, Wegene
Schartinger, Volker H.
Dudas, Jozsef
Ingruber, Julia
Greier, Maria C.
Steinbichler, Teresa B.
Laimer, Johannes
Stoiber, Heribert
Riechelmann, Herbert
Kofler, Barbara
author_sort Borena, Wegene
collection PubMed
description Background: High risk human papillomavirus (hr-HPV)-associated oropharyngeal cancers (OPCs) are characterized by significantly better therapy responses. In order to implement a de-escalated treatment strategy for this tumor entity, it is highly crucial to accurately distinguish HPV-associated OPCs from non-HPV-associated ones. Methods: In this prospective study, 56 patients with histologically confirmed OPC were evaluated. A commercially available sandwich ELISA test system was used for the detection of hr-HPV E7 oncoprotein targeting the genotypes 16, 18 and 45. Results were presented as optical density. Positivity for HPV DNA and p16 immunohistochemistry (IHC) was taken as the reference method. Results: E7 positivity was significantly associated with the reference method (p = 0.048). The sensitivity, specificity, positive predictive value and negative predictive value for the E7 oncoptotein was 60.9% (95% CI 38.5 to 80.3%), 66.7% (95% CI 46% to 83.5%), 64.2% (95% CI 49.4 to 77.4%) and 63.01% (95% CI 48.9–75.2%), respectively, for the cutoff provided by the manufacturer. Conclusions: We found a significant association between E7 oncoprotein detection and the currently used combination. We believe that the use of the ELISA based E7 antigen test could be a valuable addition in cases of ambiguous findings and may be used in combination with other techniques to distinguish between HPV-driven and non-HPV-driven OPCs. However, the low sensitivity of the assay coupled with the small sample size in our study may represent a limitation. We recommend that future larger studies elucidate the diagnostic value of the E7 brush test.
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spelling pubmed-75631712020-10-27 HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test Borena, Wegene Schartinger, Volker H. Dudas, Jozsef Ingruber, Julia Greier, Maria C. Steinbichler, Teresa B. Laimer, Johannes Stoiber, Heribert Riechelmann, Herbert Kofler, Barbara Cancers (Basel) Article Background: High risk human papillomavirus (hr-HPV)-associated oropharyngeal cancers (OPCs) are characterized by significantly better therapy responses. In order to implement a de-escalated treatment strategy for this tumor entity, it is highly crucial to accurately distinguish HPV-associated OPCs from non-HPV-associated ones. Methods: In this prospective study, 56 patients with histologically confirmed OPC were evaluated. A commercially available sandwich ELISA test system was used for the detection of hr-HPV E7 oncoprotein targeting the genotypes 16, 18 and 45. Results were presented as optical density. Positivity for HPV DNA and p16 immunohistochemistry (IHC) was taken as the reference method. Results: E7 positivity was significantly associated with the reference method (p = 0.048). The sensitivity, specificity, positive predictive value and negative predictive value for the E7 oncoptotein was 60.9% (95% CI 38.5 to 80.3%), 66.7% (95% CI 46% to 83.5%), 64.2% (95% CI 49.4 to 77.4%) and 63.01% (95% CI 48.9–75.2%), respectively, for the cutoff provided by the manufacturer. Conclusions: We found a significant association between E7 oncoprotein detection and the currently used combination. We believe that the use of the ELISA based E7 antigen test could be a valuable addition in cases of ambiguous findings and may be used in combination with other techniques to distinguish between HPV-driven and non-HPV-driven OPCs. However, the low sensitivity of the assay coupled with the small sample size in our study may represent a limitation. We recommend that future larger studies elucidate the diagnostic value of the E7 brush test. MDPI 2020-08-23 /pmc/articles/PMC7563171/ /pubmed/32842554 http://dx.doi.org/10.3390/cancers12092388 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borena, Wegene
Schartinger, Volker H.
Dudas, Jozsef
Ingruber, Julia
Greier, Maria C.
Steinbichler, Teresa B.
Laimer, Johannes
Stoiber, Heribert
Riechelmann, Herbert
Kofler, Barbara
HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title_full HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title_fullStr HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title_full_unstemmed HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title_short HPV-Induced Oropharyngeal Cancer and the Role of the E7 Oncoprotein Detection via Brush Test
title_sort hpv-induced oropharyngeal cancer and the role of the e7 oncoprotein detection via brush test
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563171/
https://www.ncbi.nlm.nih.gov/pubmed/32842554
http://dx.doi.org/10.3390/cancers12092388
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