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Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy

Micronuclei (MN) trigger antitumor immune responses via the cyclic GMP-AMP synthase-signaling effector stimulator of interferon genes (cGAS-STING) pathway. Radiotherapy induces MN in peripheral blood lymphocytes. However, data for solid tumors are lacking. Here, we analyzed MN post-radiotherapy in s...

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Autores principales: Kobayashi, Daijiro, Oike, Takahiro, Murata, Kazutoshi, Irie, Daisuke, Hirota, Yuka, Sato, Hiro, Shibata, Atsushi, Ohno, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563241/
https://www.ncbi.nlm.nih.gov/pubmed/32899112
http://dx.doi.org/10.3390/jpm10030110
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author Kobayashi, Daijiro
Oike, Takahiro
Murata, Kazutoshi
Irie, Daisuke
Hirota, Yuka
Sato, Hiro
Shibata, Atsushi
Ohno, Tatsuya
author_facet Kobayashi, Daijiro
Oike, Takahiro
Murata, Kazutoshi
Irie, Daisuke
Hirota, Yuka
Sato, Hiro
Shibata, Atsushi
Ohno, Tatsuya
author_sort Kobayashi, Daijiro
collection PubMed
description Micronuclei (MN) trigger antitumor immune responses via the cyclic GMP-AMP synthase-signaling effector stimulator of interferon genes (cGAS-STING) pathway. Radiotherapy induces MN in peripheral blood lymphocytes. However, data for solid tumors are lacking. Here, we analyzed MN post-radiotherapy in solid tumor samples. Tumor biopsy specimens were obtained from seven prospectively recruited patients with cervical cancer, before treatment and after receiving radiotherapy at a dose of 10 Gy (in five fractions). The samples were stained with 4′,6-diamidino-2-phenylindole dihydrochloride, and 200 nuclei per sample were randomly identified and assessed for the presence of MN or apoptosis, based on nuclear morphology. The median number of MN-harboring nuclei was significantly greater in samples from patients treated with radiotherapy than in pre-treatment samples (151 (range, 16–327) versus 28 (range, 0–61); p = 0.015). No significant differences in the number of apoptotic nuclei were observed between pre-treatment and 10 Gy samples (5 (range, 0–30) versus 12 (range, 2–30); p = 0.30). This is the first report to demonstrate MN induction by radiotherapy in solid tumors. The results provide clinical evidence of the activation of antitumor immune responses by radiotherapy.
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spelling pubmed-75632412020-10-27 Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy Kobayashi, Daijiro Oike, Takahiro Murata, Kazutoshi Irie, Daisuke Hirota, Yuka Sato, Hiro Shibata, Atsushi Ohno, Tatsuya J Pers Med Communication Micronuclei (MN) trigger antitumor immune responses via the cyclic GMP-AMP synthase-signaling effector stimulator of interferon genes (cGAS-STING) pathway. Radiotherapy induces MN in peripheral blood lymphocytes. However, data for solid tumors are lacking. Here, we analyzed MN post-radiotherapy in solid tumor samples. Tumor biopsy specimens were obtained from seven prospectively recruited patients with cervical cancer, before treatment and after receiving radiotherapy at a dose of 10 Gy (in five fractions). The samples were stained with 4′,6-diamidino-2-phenylindole dihydrochloride, and 200 nuclei per sample were randomly identified and assessed for the presence of MN or apoptosis, based on nuclear morphology. The median number of MN-harboring nuclei was significantly greater in samples from patients treated with radiotherapy than in pre-treatment samples (151 (range, 16–327) versus 28 (range, 0–61); p = 0.015). No significant differences in the number of apoptotic nuclei were observed between pre-treatment and 10 Gy samples (5 (range, 0–30) versus 12 (range, 2–30); p = 0.30). This is the first report to demonstrate MN induction by radiotherapy in solid tumors. The results provide clinical evidence of the activation of antitumor immune responses by radiotherapy. MDPI 2020-09-03 /pmc/articles/PMC7563241/ /pubmed/32899112 http://dx.doi.org/10.3390/jpm10030110 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kobayashi, Daijiro
Oike, Takahiro
Murata, Kazutoshi
Irie, Daisuke
Hirota, Yuka
Sato, Hiro
Shibata, Atsushi
Ohno, Tatsuya
Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title_full Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title_fullStr Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title_full_unstemmed Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title_short Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
title_sort induction of micronuclei in cervical cancer treated with radiotherapy
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563241/
https://www.ncbi.nlm.nih.gov/pubmed/32899112
http://dx.doi.org/10.3390/jpm10030110
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