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ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA
Lamins are important filaments forming the inner nuclear membrane. Lamin A is processed by zinc metalloproteinase (ZMPSTE24). Failure to cleave a truncated form of prelamin A—also called progerin—causes Hutchinson–Gilford progeria syndrome a well-known premature aging disease. Minor levels of proger...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563344/ https://www.ncbi.nlm.nih.gov/pubmed/32872320 http://dx.doi.org/10.3390/cells9091981 |
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author | Messner, Moritz Ghadge, Santhosh Kumar Maurer, Thomas Graber, Michael Staggl, Simon Christine Maier, Sarah Pölzl, Gerhard Zaruba, Marc-Michael |
author_facet | Messner, Moritz Ghadge, Santhosh Kumar Maurer, Thomas Graber, Michael Staggl, Simon Christine Maier, Sarah Pölzl, Gerhard Zaruba, Marc-Michael |
author_sort | Messner, Moritz |
collection | PubMed |
description | Lamins are important filaments forming the inner nuclear membrane. Lamin A is processed by zinc metalloproteinase (ZMPSTE24). Failure to cleave a truncated form of prelamin A—also called progerin—causes Hutchinson–Gilford progeria syndrome a well-known premature aging disease. Minor levels of progerin are readily expressed in the blood of healthy individuals due to alternative splicing. Previously, we found an association of increased progerin mRNA with overweight and chronic inflammation (hs-CRP). Here, we aimed to elucidate correlations of ZMPSTE24, lamin A/C and progerin with the inflammatory marker hs-CRP. In this retrospective, cross-sectional study we analyzed blood samples from 110 heart failure patients for quantitative mRNA expression of ZMPSTE24, lamin A/C, progerin and hs-CRP protein. Spearman correlations and linear regression analyses including adjustments for age, gender and ejection fraction showed a significant positive correlation of lnprogerin with lnZMPSTE24 (n = 110; r = 0.33; p = 0.0004) and lnlamin A/C (n = 110; r = 0.82, p < 0.0001), whereas no association was observed between lnlamin A/C and lnZMPSTE24 expression. Further analyses showed a significant positive correlation of lnhs-CRP with lnZMPSTE24 (n = 110; r = 0.21; p = 0.01) and lnlamin A/C (n = 110; r = 0.24; p = 0.03). We conclude that chronic inflammation is associated with increased expression of ZMPSTE24 and lamin A/C mRNA. Both markers also positively correlate with increased expression of the premature aging marker progerin which may be linked to cardiovascular aging. |
format | Online Article Text |
id | pubmed-7563344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75633442020-10-27 ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA Messner, Moritz Ghadge, Santhosh Kumar Maurer, Thomas Graber, Michael Staggl, Simon Christine Maier, Sarah Pölzl, Gerhard Zaruba, Marc-Michael Cells Article Lamins are important filaments forming the inner nuclear membrane. Lamin A is processed by zinc metalloproteinase (ZMPSTE24). Failure to cleave a truncated form of prelamin A—also called progerin—causes Hutchinson–Gilford progeria syndrome a well-known premature aging disease. Minor levels of progerin are readily expressed in the blood of healthy individuals due to alternative splicing. Previously, we found an association of increased progerin mRNA with overweight and chronic inflammation (hs-CRP). Here, we aimed to elucidate correlations of ZMPSTE24, lamin A/C and progerin with the inflammatory marker hs-CRP. In this retrospective, cross-sectional study we analyzed blood samples from 110 heart failure patients for quantitative mRNA expression of ZMPSTE24, lamin A/C, progerin and hs-CRP protein. Spearman correlations and linear regression analyses including adjustments for age, gender and ejection fraction showed a significant positive correlation of lnprogerin with lnZMPSTE24 (n = 110; r = 0.33; p = 0.0004) and lnlamin A/C (n = 110; r = 0.82, p < 0.0001), whereas no association was observed between lnlamin A/C and lnZMPSTE24 expression. Further analyses showed a significant positive correlation of lnhs-CRP with lnZMPSTE24 (n = 110; r = 0.21; p = 0.01) and lnlamin A/C (n = 110; r = 0.24; p = 0.03). We conclude that chronic inflammation is associated with increased expression of ZMPSTE24 and lamin A/C mRNA. Both markers also positively correlate with increased expression of the premature aging marker progerin which may be linked to cardiovascular aging. MDPI 2020-08-28 /pmc/articles/PMC7563344/ /pubmed/32872320 http://dx.doi.org/10.3390/cells9091981 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Messner, Moritz Ghadge, Santhosh Kumar Maurer, Thomas Graber, Michael Staggl, Simon Christine Maier, Sarah Pölzl, Gerhard Zaruba, Marc-Michael ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title | ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title_full | ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title_fullStr | ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title_full_unstemmed | ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title_short | ZMPSTE24 Is Associated with Elevated Inflammation and Progerin mRNA |
title_sort | zmpste24 is associated with elevated inflammation and progerin mrna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563344/ https://www.ncbi.nlm.nih.gov/pubmed/32872320 http://dx.doi.org/10.3390/cells9091981 |
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