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Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae

Surfactant protein A (SP-A) plays an important role in innate immunity. The sex-dependent survival of infected SP-A knockout (KO) mice has been observed. Our goal was to study the impact of ozone (O(3)) and sex, as well as gonadal hormones, on the bronchoalveolar lavage (BAL) readouts and survival,...

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Autores principales: Gandhi, Chintan K., Mikerov, Anatoly N., Durrani, Faryal, Umstead, Todd M., Hu, Sanmei, Wang, Guirong, Phelps, David S., Floros, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563396/
https://www.ncbi.nlm.nih.gov/pubmed/32899781
http://dx.doi.org/10.3390/microorganisms8091354
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author Gandhi, Chintan K.
Mikerov, Anatoly N.
Durrani, Faryal
Umstead, Todd M.
Hu, Sanmei
Wang, Guirong
Phelps, David S.
Floros, Joanna
author_facet Gandhi, Chintan K.
Mikerov, Anatoly N.
Durrani, Faryal
Umstead, Todd M.
Hu, Sanmei
Wang, Guirong
Phelps, David S.
Floros, Joanna
author_sort Gandhi, Chintan K.
collection PubMed
description Surfactant protein A (SP-A) plays an important role in innate immunity. The sex-dependent survival of infected SP-A knockout (KO) mice has been observed. Our goal was to study the impact of ozone (O(3)) and sex, as well as gonadal hormones, on the bronchoalveolar lavage (BAL) readouts and survival, respectively, of Klebsiella pneumoniae-infected SP-A KO mice. Male and female SP-A KO mice were exposed to O(3) or filtered air and infected with K. pneumoniae. We studied markers of inflammation and tissue damage at 4, 24, and 48 h, as well as the survival over 14 days, of gonadectomized (Gx) mice implanted with control pellets (CoP) or hormone (5α-dihydrotestosterone (DHT) in female gonadectomized mice (GxF) or 17β-estradiol (E(2)) in male gonadectomized mice (GxM)). We observed: (1) an increase in neutrophil and macrophage inflammatory protein-2 levels as time progressed post-infection, and O(3) exposure appeared to increase this response; (2) an increase in lactate dehydrogenase, total protein, oxidized protein, and phospholipids in response to O(3) with no consistent sex differences in studied parameters; and (3) a reduction in survival of the GxM and CoP mice, the GxM and E(2) mice, and the GxF and DHT mice but not for the GxF and CoP mice after O(3). Without SP-A, (a) sex was found to have a minimal impact on BAL cellular composition and tissue damage markers, and (b) the impact of gonadal hormones on survival was found to involve different mechanisms than in the presence of SP-A.
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spelling pubmed-75633962020-10-27 Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae Gandhi, Chintan K. Mikerov, Anatoly N. Durrani, Faryal Umstead, Todd M. Hu, Sanmei Wang, Guirong Phelps, David S. Floros, Joanna Microorganisms Article Surfactant protein A (SP-A) plays an important role in innate immunity. The sex-dependent survival of infected SP-A knockout (KO) mice has been observed. Our goal was to study the impact of ozone (O(3)) and sex, as well as gonadal hormones, on the bronchoalveolar lavage (BAL) readouts and survival, respectively, of Klebsiella pneumoniae-infected SP-A KO mice. Male and female SP-A KO mice were exposed to O(3) or filtered air and infected with K. pneumoniae. We studied markers of inflammation and tissue damage at 4, 24, and 48 h, as well as the survival over 14 days, of gonadectomized (Gx) mice implanted with control pellets (CoP) or hormone (5α-dihydrotestosterone (DHT) in female gonadectomized mice (GxF) or 17β-estradiol (E(2)) in male gonadectomized mice (GxM)). We observed: (1) an increase in neutrophil and macrophage inflammatory protein-2 levels as time progressed post-infection, and O(3) exposure appeared to increase this response; (2) an increase in lactate dehydrogenase, total protein, oxidized protein, and phospholipids in response to O(3) with no consistent sex differences in studied parameters; and (3) a reduction in survival of the GxM and CoP mice, the GxM and E(2) mice, and the GxF and DHT mice but not for the GxF and CoP mice after O(3). Without SP-A, (a) sex was found to have a minimal impact on BAL cellular composition and tissue damage markers, and (b) the impact of gonadal hormones on survival was found to involve different mechanisms than in the presence of SP-A. MDPI 2020-09-04 /pmc/articles/PMC7563396/ /pubmed/32899781 http://dx.doi.org/10.3390/microorganisms8091354 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gandhi, Chintan K.
Mikerov, Anatoly N.
Durrani, Faryal
Umstead, Todd M.
Hu, Sanmei
Wang, Guirong
Phelps, David S.
Floros, Joanna
Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title_full Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title_fullStr Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title_full_unstemmed Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title_short Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae
title_sort impact of ozone, sex, and gonadal hormones on bronchoalveolar lavage characteristics and survival in sp-a ko mice infected with klebsiella pneumoniae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563396/
https://www.ncbi.nlm.nih.gov/pubmed/32899781
http://dx.doi.org/10.3390/microorganisms8091354
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