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Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells

Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D;...

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Autores principales: Dechaumes, Arthur, Bertin, Antoine, Sane, Famara, Levet, Sandrine, Varghese, Jennifer, Charvet, Benjamin, Gmyr, Valéry, Kerr-Conte, Julie, Pierquin, Justine, Arunkumar, Govindakarnavar, Pattou, François, Perron, Hervé, Hober, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563422/
https://www.ncbi.nlm.nih.gov/pubmed/32883004
http://dx.doi.org/10.3390/microorganisms8091335
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author Dechaumes, Arthur
Bertin, Antoine
Sane, Famara
Levet, Sandrine
Varghese, Jennifer
Charvet, Benjamin
Gmyr, Valéry
Kerr-Conte, Julie
Pierquin, Justine
Arunkumar, Govindakarnavar
Pattou, François
Perron, Hervé
Hober, Didier
author_facet Dechaumes, Arthur
Bertin, Antoine
Sane, Famara
Levet, Sandrine
Varghese, Jennifer
Charvet, Benjamin
Gmyr, Valéry
Kerr-Conte, Julie
Pierquin, Justine
Arunkumar, Govindakarnavar
Pattou, François
Perron, Hervé
Hober, Didier
author_sort Dechaumes, Arthur
collection PubMed
description Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D; specifically, coxsackievirus-B (CV-B) has been detected in the blood and pancreas of patients with T1D. Notably, viruses can activate HERV-W expression. Hence, we evaluated the effect of CV-B4 infection on HERV-W ENV mRNA expression. Primary human pancreatic ductal cells were obtained from five brain-dead donors. In the pancreatic cells of three donors, the HERV-W ENV mRNA level measured using RT-qPCR was upregulated upon CV-B4 infection. The HERV-W ENV protein was detected in the infected cells using the immunoblot assay. In human PBMCs inoculated with CV-B4 or when CV-B4 was incubated with an enhancing serum, the HERV-W ENV mRNA level was higher than the background RNA level. In monocyte-derived macrophages obtained from 5 of 13 donors, the HERV-W ENV mRNA level was higher in cultures inoculated with CV-B4 than in the control. Therefore, CV-B4 can upregulate or induce the transcription of a certain HERV-W ENV copy (or copies) in primary cell cultures, such as monocytes, macrophages, and pancreatic cells.
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spelling pubmed-75634222020-10-27 Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells Dechaumes, Arthur Bertin, Antoine Sane, Famara Levet, Sandrine Varghese, Jennifer Charvet, Benjamin Gmyr, Valéry Kerr-Conte, Julie Pierquin, Justine Arunkumar, Govindakarnavar Pattou, François Perron, Hervé Hober, Didier Microorganisms Article Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D; specifically, coxsackievirus-B (CV-B) has been detected in the blood and pancreas of patients with T1D. Notably, viruses can activate HERV-W expression. Hence, we evaluated the effect of CV-B4 infection on HERV-W ENV mRNA expression. Primary human pancreatic ductal cells were obtained from five brain-dead donors. In the pancreatic cells of three donors, the HERV-W ENV mRNA level measured using RT-qPCR was upregulated upon CV-B4 infection. The HERV-W ENV protein was detected in the infected cells using the immunoblot assay. In human PBMCs inoculated with CV-B4 or when CV-B4 was incubated with an enhancing serum, the HERV-W ENV mRNA level was higher than the background RNA level. In monocyte-derived macrophages obtained from 5 of 13 donors, the HERV-W ENV mRNA level was higher in cultures inoculated with CV-B4 than in the control. Therefore, CV-B4 can upregulate or induce the transcription of a certain HERV-W ENV copy (or copies) in primary cell cultures, such as monocytes, macrophages, and pancreatic cells. MDPI 2020-09-01 /pmc/articles/PMC7563422/ /pubmed/32883004 http://dx.doi.org/10.3390/microorganisms8091335 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dechaumes, Arthur
Bertin, Antoine
Sane, Famara
Levet, Sandrine
Varghese, Jennifer
Charvet, Benjamin
Gmyr, Valéry
Kerr-Conte, Julie
Pierquin, Justine
Arunkumar, Govindakarnavar
Pattou, François
Perron, Hervé
Hober, Didier
Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title_full Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title_fullStr Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title_full_unstemmed Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title_short Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
title_sort coxsackievirus-b4 infection can induce the expression of human endogenous retrovirus w in primary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563422/
https://www.ncbi.nlm.nih.gov/pubmed/32883004
http://dx.doi.org/10.3390/microorganisms8091335
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