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Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells
Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D;...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563422/ https://www.ncbi.nlm.nih.gov/pubmed/32883004 http://dx.doi.org/10.3390/microorganisms8091335 |
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author | Dechaumes, Arthur Bertin, Antoine Sane, Famara Levet, Sandrine Varghese, Jennifer Charvet, Benjamin Gmyr, Valéry Kerr-Conte, Julie Pierquin, Justine Arunkumar, Govindakarnavar Pattou, François Perron, Hervé Hober, Didier |
author_facet | Dechaumes, Arthur Bertin, Antoine Sane, Famara Levet, Sandrine Varghese, Jennifer Charvet, Benjamin Gmyr, Valéry Kerr-Conte, Julie Pierquin, Justine Arunkumar, Govindakarnavar Pattou, François Perron, Hervé Hober, Didier |
author_sort | Dechaumes, Arthur |
collection | PubMed |
description | Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D; specifically, coxsackievirus-B (CV-B) has been detected in the blood and pancreas of patients with T1D. Notably, viruses can activate HERV-W expression. Hence, we evaluated the effect of CV-B4 infection on HERV-W ENV mRNA expression. Primary human pancreatic ductal cells were obtained from five brain-dead donors. In the pancreatic cells of three donors, the HERV-W ENV mRNA level measured using RT-qPCR was upregulated upon CV-B4 infection. The HERV-W ENV protein was detected in the infected cells using the immunoblot assay. In human PBMCs inoculated with CV-B4 or when CV-B4 was incubated with an enhancing serum, the HERV-W ENV mRNA level was higher than the background RNA level. In monocyte-derived macrophages obtained from 5 of 13 donors, the HERV-W ENV mRNA level was higher in cultures inoculated with CV-B4 than in the control. Therefore, CV-B4 can upregulate or induce the transcription of a certain HERV-W ENV copy (or copies) in primary cell cultures, such as monocytes, macrophages, and pancreatic cells. |
format | Online Article Text |
id | pubmed-7563422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75634222020-10-27 Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells Dechaumes, Arthur Bertin, Antoine Sane, Famara Levet, Sandrine Varghese, Jennifer Charvet, Benjamin Gmyr, Valéry Kerr-Conte, Julie Pierquin, Justine Arunkumar, Govindakarnavar Pattou, François Perron, Hervé Hober, Didier Microorganisms Article Human Endogenous Retrovirus W Envelope (HERV-W ENV) mRNA or protein can be found in peripheral blood mononuclear cells (PBMCs) and exocrine pancreas of patients with type 1 diabetes (T1D). Further, previous observations have shown an association between enteroviral infection and development of T1D; specifically, coxsackievirus-B (CV-B) has been detected in the blood and pancreas of patients with T1D. Notably, viruses can activate HERV-W expression. Hence, we evaluated the effect of CV-B4 infection on HERV-W ENV mRNA expression. Primary human pancreatic ductal cells were obtained from five brain-dead donors. In the pancreatic cells of three donors, the HERV-W ENV mRNA level measured using RT-qPCR was upregulated upon CV-B4 infection. The HERV-W ENV protein was detected in the infected cells using the immunoblot assay. In human PBMCs inoculated with CV-B4 or when CV-B4 was incubated with an enhancing serum, the HERV-W ENV mRNA level was higher than the background RNA level. In monocyte-derived macrophages obtained from 5 of 13 donors, the HERV-W ENV mRNA level was higher in cultures inoculated with CV-B4 than in the control. Therefore, CV-B4 can upregulate or induce the transcription of a certain HERV-W ENV copy (or copies) in primary cell cultures, such as monocytes, macrophages, and pancreatic cells. MDPI 2020-09-01 /pmc/articles/PMC7563422/ /pubmed/32883004 http://dx.doi.org/10.3390/microorganisms8091335 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dechaumes, Arthur Bertin, Antoine Sane, Famara Levet, Sandrine Varghese, Jennifer Charvet, Benjamin Gmyr, Valéry Kerr-Conte, Julie Pierquin, Justine Arunkumar, Govindakarnavar Pattou, François Perron, Hervé Hober, Didier Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title | Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title_full | Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title_fullStr | Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title_full_unstemmed | Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title_short | Coxsackievirus-B4 Infection Can Induce the Expression of Human Endogenous Retrovirus W in Primary Cells |
title_sort | coxsackievirus-b4 infection can induce the expression of human endogenous retrovirus w in primary cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563422/ https://www.ncbi.nlm.nih.gov/pubmed/32883004 http://dx.doi.org/10.3390/microorganisms8091335 |
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