Cargando…

Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure

Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or wi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lai, Lilin, Ault, Kevin, Rouphael, Nadine, Beck, Allison, Domjahn, Briyana, Xu, Yongxian, Anderson, Evan J., Cheng, Andrew, Nakamura, Aya, Hoagland, Rebecca J., Kelley, Colleen, Edupuganti, Srilatha, Mask, Karen, Nesin, Mirjana, Unger, Elizabeth R., Panicker, Gitika, David, Hagit, Mulligan, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563427/
https://www.ncbi.nlm.nih.gov/pubmed/32629943
http://dx.doi.org/10.3390/vaccines8030348
_version_ 1783595487516950528
author Lai, Lilin
Ault, Kevin
Rouphael, Nadine
Beck, Allison
Domjahn, Briyana
Xu, Yongxian
Anderson, Evan J.
Cheng, Andrew
Nakamura, Aya
Hoagland, Rebecca J.
Kelley, Colleen
Edupuganti, Srilatha
Mask, Karen
Nesin, Mirjana
Unger, Elizabeth R.
Panicker, Gitika
David, Hagit
Mulligan, Mark J.
author_facet Lai, Lilin
Ault, Kevin
Rouphael, Nadine
Beck, Allison
Domjahn, Briyana
Xu, Yongxian
Anderson, Evan J.
Cheng, Andrew
Nakamura, Aya
Hoagland, Rebecca J.
Kelley, Colleen
Edupuganti, Srilatha
Mask, Karen
Nesin, Mirjana
Unger, Elizabeth R.
Panicker, Gitika
David, Hagit
Mulligan, Mark J.
author_sort Lai, Lilin
collection PubMed
description Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or without prior exposure with type 16 and 18 HPV. In a prospective cohort, vaccine naïve females received three doses of 4vHPV vaccine and were followed for two years to assess cellular (intracellular cytokine staining, proliferation and B cell ELISpot assays) and humoral (multiplex L1/L2 viral-like particles (VLP) and M4 ELISAs) responses. Frequencies of vaccine-specific CD4+ T cells correlated with antibody responses. Higher HPV antibody titers were found at all time points in participants previously exposed to HPV, except for anti-HPV-18 at Day 187 (one week post the third vaccination). Retrospective cohorts enrolled females who had previously received two or three 4vHPV doses and tested antibody titers by M4 ELISA and pseudovirion neutralization assay along with memory B cells (MBCs). Almost all women enrolled in a retrospective cohort with two prior doses and all women enrolled in a retrospective cohort with three prior doses had sustained antibody and memory responses. Our findings indicate that HPV vaccination induces a long-lasting, robust cellular and humoral immune responses.
format Online
Article
Text
id pubmed-7563427
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75634272020-10-27 Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure Lai, Lilin Ault, Kevin Rouphael, Nadine Beck, Allison Domjahn, Briyana Xu, Yongxian Anderson, Evan J. Cheng, Andrew Nakamura, Aya Hoagland, Rebecca J. Kelley, Colleen Edupuganti, Srilatha Mask, Karen Nesin, Mirjana Unger, Elizabeth R. Panicker, Gitika David, Hagit Mulligan, Mark J. Vaccines (Basel) Article Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or without prior exposure with type 16 and 18 HPV. In a prospective cohort, vaccine naïve females received three doses of 4vHPV vaccine and were followed for two years to assess cellular (intracellular cytokine staining, proliferation and B cell ELISpot assays) and humoral (multiplex L1/L2 viral-like particles (VLP) and M4 ELISAs) responses. Frequencies of vaccine-specific CD4+ T cells correlated with antibody responses. Higher HPV antibody titers were found at all time points in participants previously exposed to HPV, except for anti-HPV-18 at Day 187 (one week post the third vaccination). Retrospective cohorts enrolled females who had previously received two or three 4vHPV doses and tested antibody titers by M4 ELISA and pseudovirion neutralization assay along with memory B cells (MBCs). Almost all women enrolled in a retrospective cohort with two prior doses and all women enrolled in a retrospective cohort with three prior doses had sustained antibody and memory responses. Our findings indicate that HPV vaccination induces a long-lasting, robust cellular and humoral immune responses. MDPI 2020-06-30 /pmc/articles/PMC7563427/ /pubmed/32629943 http://dx.doi.org/10.3390/vaccines8030348 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Lilin
Ault, Kevin
Rouphael, Nadine
Beck, Allison
Domjahn, Briyana
Xu, Yongxian
Anderson, Evan J.
Cheng, Andrew
Nakamura, Aya
Hoagland, Rebecca J.
Kelley, Colleen
Edupuganti, Srilatha
Mask, Karen
Nesin, Mirjana
Unger, Elizabeth R.
Panicker, Gitika
David, Hagit
Mulligan, Mark J.
Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title_full Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title_fullStr Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title_full_unstemmed Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title_short Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
title_sort duration of cellular and humoral responses after quadrivalent human papillomavirus vaccination in healthy female adults with or without prior type 16 and/or 18 exposure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563427/
https://www.ncbi.nlm.nih.gov/pubmed/32629943
http://dx.doi.org/10.3390/vaccines8030348
work_keys_str_mv AT laililin durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT aultkevin durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT rouphaelnadine durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT beckallison durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT domjahnbriyana durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT xuyongxian durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT andersonevanj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT chengandrew durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT nakamuraaya durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT hoaglandrebeccaj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT kelleycolleen durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT edupugantisrilatha durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT maskkaren durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT nesinmirjana durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT ungerelizabethr durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT panickergitika durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT davidhagit durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure
AT mulliganmarkj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure