Cargando…
Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure
Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or wi...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563427/ https://www.ncbi.nlm.nih.gov/pubmed/32629943 http://dx.doi.org/10.3390/vaccines8030348 |
_version_ | 1783595487516950528 |
---|---|
author | Lai, Lilin Ault, Kevin Rouphael, Nadine Beck, Allison Domjahn, Briyana Xu, Yongxian Anderson, Evan J. Cheng, Andrew Nakamura, Aya Hoagland, Rebecca J. Kelley, Colleen Edupuganti, Srilatha Mask, Karen Nesin, Mirjana Unger, Elizabeth R. Panicker, Gitika David, Hagit Mulligan, Mark J. |
author_facet | Lai, Lilin Ault, Kevin Rouphael, Nadine Beck, Allison Domjahn, Briyana Xu, Yongxian Anderson, Evan J. Cheng, Andrew Nakamura, Aya Hoagland, Rebecca J. Kelley, Colleen Edupuganti, Srilatha Mask, Karen Nesin, Mirjana Unger, Elizabeth R. Panicker, Gitika David, Hagit Mulligan, Mark J. |
author_sort | Lai, Lilin |
collection | PubMed |
description | Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or without prior exposure with type 16 and 18 HPV. In a prospective cohort, vaccine naïve females received three doses of 4vHPV vaccine and were followed for two years to assess cellular (intracellular cytokine staining, proliferation and B cell ELISpot assays) and humoral (multiplex L1/L2 viral-like particles (VLP) and M4 ELISAs) responses. Frequencies of vaccine-specific CD4+ T cells correlated with antibody responses. Higher HPV antibody titers were found at all time points in participants previously exposed to HPV, except for anti-HPV-18 at Day 187 (one week post the third vaccination). Retrospective cohorts enrolled females who had previously received two or three 4vHPV doses and tested antibody titers by M4 ELISA and pseudovirion neutralization assay along with memory B cells (MBCs). Almost all women enrolled in a retrospective cohort with two prior doses and all women enrolled in a retrospective cohort with three prior doses had sustained antibody and memory responses. Our findings indicate that HPV vaccination induces a long-lasting, robust cellular and humoral immune responses. |
format | Online Article Text |
id | pubmed-7563427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75634272020-10-27 Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure Lai, Lilin Ault, Kevin Rouphael, Nadine Beck, Allison Domjahn, Briyana Xu, Yongxian Anderson, Evan J. Cheng, Andrew Nakamura, Aya Hoagland, Rebecca J. Kelley, Colleen Edupuganti, Srilatha Mask, Karen Nesin, Mirjana Unger, Elizabeth R. Panicker, Gitika David, Hagit Mulligan, Mark J. Vaccines (Basel) Article Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or without prior exposure with type 16 and 18 HPV. In a prospective cohort, vaccine naïve females received three doses of 4vHPV vaccine and were followed for two years to assess cellular (intracellular cytokine staining, proliferation and B cell ELISpot assays) and humoral (multiplex L1/L2 viral-like particles (VLP) and M4 ELISAs) responses. Frequencies of vaccine-specific CD4+ T cells correlated with antibody responses. Higher HPV antibody titers were found at all time points in participants previously exposed to HPV, except for anti-HPV-18 at Day 187 (one week post the third vaccination). Retrospective cohorts enrolled females who had previously received two or three 4vHPV doses and tested antibody titers by M4 ELISA and pseudovirion neutralization assay along with memory B cells (MBCs). Almost all women enrolled in a retrospective cohort with two prior doses and all women enrolled in a retrospective cohort with three prior doses had sustained antibody and memory responses. Our findings indicate that HPV vaccination induces a long-lasting, robust cellular and humoral immune responses. MDPI 2020-06-30 /pmc/articles/PMC7563427/ /pubmed/32629943 http://dx.doi.org/10.3390/vaccines8030348 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lai, Lilin Ault, Kevin Rouphael, Nadine Beck, Allison Domjahn, Briyana Xu, Yongxian Anderson, Evan J. Cheng, Andrew Nakamura, Aya Hoagland, Rebecca J. Kelley, Colleen Edupuganti, Srilatha Mask, Karen Nesin, Mirjana Unger, Elizabeth R. Panicker, Gitika David, Hagit Mulligan, Mark J. Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title | Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title_full | Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title_fullStr | Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title_full_unstemmed | Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title_short | Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure |
title_sort | duration of cellular and humoral responses after quadrivalent human papillomavirus vaccination in healthy female adults with or without prior type 16 and/or 18 exposure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563427/ https://www.ncbi.nlm.nih.gov/pubmed/32629943 http://dx.doi.org/10.3390/vaccines8030348 |
work_keys_str_mv | AT laililin durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT aultkevin durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT rouphaelnadine durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT beckallison durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT domjahnbriyana durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT xuyongxian durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT andersonevanj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT chengandrew durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT nakamuraaya durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT hoaglandrebeccaj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT kelleycolleen durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT edupugantisrilatha durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT maskkaren durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT nesinmirjana durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT ungerelizabethr durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT panickergitika durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT davidhagit durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure AT mulliganmarkj durationofcellularandhumoralresponsesafterquadrivalenthumanpapillomavirusvaccinationinhealthyfemaleadultswithorwithoutpriortype16andor18exposure |