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Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical co...

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Autores principales: Yusoff, Siti Fairuz, Haron, Farah Farhanah, Tengku Muda Mohamed, Mahmud, Asib, Norhayu, Sakimin, Siti Zaharah, Abu Kassim, Faizah, Ismail, Siti Izera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563699/
https://www.ncbi.nlm.nih.gov/pubmed/32932993
http://dx.doi.org/10.3390/biology9090286
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author Yusoff, Siti Fairuz
Haron, Farah Farhanah
Tengku Muda Mohamed, Mahmud
Asib, Norhayu
Sakimin, Siti Zaharah
Abu Kassim, Faizah
Ismail, Siti Izera
author_facet Yusoff, Siti Fairuz
Haron, Farah Farhanah
Tengku Muda Mohamed, Mahmud
Asib, Norhayu
Sakimin, Siti Zaharah
Abu Kassim, Faizah
Ismail, Siti Izera
author_sort Yusoff, Siti Fairuz
collection PubMed
description Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.
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spelling pubmed-75636992020-10-27 Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits Yusoff, Siti Fairuz Haron, Farah Farhanah Tengku Muda Mohamed, Mahmud Asib, Norhayu Sakimin, Siti Zaharah Abu Kassim, Faizah Ismail, Siti Izera Biology (Basel) Article Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent. MDPI 2020-09-11 /pmc/articles/PMC7563699/ /pubmed/32932993 http://dx.doi.org/10.3390/biology9090286 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yusoff, Siti Fairuz
Haron, Farah Farhanah
Tengku Muda Mohamed, Mahmud
Asib, Norhayu
Sakimin, Siti Zaharah
Abu Kassim, Faizah
Ismail, Siti Izera
Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title_full Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title_fullStr Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title_full_unstemmed Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title_short Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits
title_sort antifungal activity and phytochemical screening of vernonia amygdalina extract against botrytis cinerea causing gray mold disease on tomato fruits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563699/
https://www.ncbi.nlm.nih.gov/pubmed/32932993
http://dx.doi.org/10.3390/biology9090286
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