Knockdown of the Trehalose-6-Phosphate Synthase Gene Using RNA Interference Inhibits Synthesis of Trehalose and Increases Lethality Rate in Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae)

SIMPLE SUMMARY: In this study, we cloned and characterized a trehalose-6-phosphate synthase (TPS) gene from D. citri (DcTPS) for the first time. Meanwhile, we used RNA interference (RNAi) technology to efficiently disrupt DcTPS gene function in order to elucidate its role in the growth and development of D. citri. Our results suggest that dsRNA-mediated gene-specific silencing resulted in a strong reduction in relative expression of DcTPS and survival rate of nymphs, as well as an increase in malformation. This work was undertaken to establish a foundation for further research on the functions of D. citri trehalose-6-phosphate synthase. This will provide a new target for the control of D. citri in the field. ABSTRACT: Diaphorina citri Kuwayama is the vector of citrus “huanglongbing”, a citrus disease which poses a significant threat to the global citrus industry. Trehalose-6-phosphate synthase (TPS) plays an important role in the regulation of trehalose levels of insects, while its functions in D. citri are unclear. In this study, full-length cDNA sequences of the TPS gene from D. citri (DcTPS) were cloned and its expression patterns at various developmental stages were investigated. The results indicated that DcTPS mRNA was expressed at each developmental stage and the highest DcTPS expression was found in the fifth-instar nymphs of D. citri. Additionally, mortality and deformity of D. citri were observed after 24 and 48 h by feeding with three different dsRNA concentrations (20, 100 and 500 ng/μL). The results indicated that DcTPS expression was declined, and mortality and malformation in nymphs were increased via feeding with dsDcTPS. Moreover, the enzyme and trehalose content were decreased, while the content of glucose was significantly higher than that of untreated (control) individuals. This suggests that DcTPS might be vital for the growth and development of D. citri and further studies of the genes should be related to molting and metabolism for controlling D. citri.