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Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling
The aim of this study was to investigate claudin-7 (CLDN7) expression in salivary adenoid cystic carcinoma (SACC) and its function in SACC cells. We determined CLDN7 expression in SACC tumors via immunohistochemistry and western blotting and evaluated the association between CLDN7 expression and cli...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563826/ https://www.ncbi.nlm.nih.gov/pubmed/32749148 http://dx.doi.org/10.1177/0963689720943583 |
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author | Ji, Huan Ding, Xu Zhang, Wei Zheng, Yang Du, Hongming Zheng, Yangyu Song, Haiyang Li, Meng Jiang, Yue Xie, Jiaxiang Wu, Ming Jiao, Pengfei Wang, Zeyu Wu, Heming Zhong, Yi |
author_facet | Ji, Huan Ding, Xu Zhang, Wei Zheng, Yang Du, Hongming Zheng, Yangyu Song, Haiyang Li, Meng Jiang, Yue Xie, Jiaxiang Wu, Ming Jiao, Pengfei Wang, Zeyu Wu, Heming Zhong, Yi |
author_sort | Ji, Huan |
collection | PubMed |
description | The aim of this study was to investigate claudin-7 (CLDN7) expression in salivary adenoid cystic carcinoma (SACC) and its function in SACC cells. We determined CLDN7 expression in SACC tumors via immunohistochemistry and western blotting and evaluated the association between CLDN7 expression and clinicopathologic variables. Besides this, we constructed a stably transfected CLDN7 knockdown SACC-LM cell line via RNAi and assessed its biological behavior changes (cell viability, migration, and invasion). The correlation between CLDN7 and epithelial-mesenchymal transition (EMT) was analyzed. Additionally, a subcutaneous tumor formation model was used to assess SACC-LM cells tumorigenicity after the CLDN7 knockdown. In the present study, we found the CLDN7 expression of tumor group was lower than that in normal salivary glands and was significantly correlated with lymph node metastasis, recurrence, and gender. CLDN7 knockdown could add the proliferation and metastasis ability of SACC by regulating EMT through Wnt/β-catenin signaling pathway. In addition, CLDN7 knockdown in SACC promoted tumor growth in nude mice. CLDN7 inhibits cell proliferation and metastasis by inactivating the Wnt/β-catenin signaling in SACC. Thus, CLDN7 expression might be a useful marker to identify the potential for progression in SACC. |
format | Online Article Text |
id | pubmed-7563826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-75638262020-10-26 Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling Ji, Huan Ding, Xu Zhang, Wei Zheng, Yang Du, Hongming Zheng, Yangyu Song, Haiyang Li, Meng Jiang, Yue Xie, Jiaxiang Wu, Ming Jiao, Pengfei Wang, Zeyu Wu, Heming Zhong, Yi Cell Transplant Cancer Pharmacogenomics and Target Therapy The aim of this study was to investigate claudin-7 (CLDN7) expression in salivary adenoid cystic carcinoma (SACC) and its function in SACC cells. We determined CLDN7 expression in SACC tumors via immunohistochemistry and western blotting and evaluated the association between CLDN7 expression and clinicopathologic variables. Besides this, we constructed a stably transfected CLDN7 knockdown SACC-LM cell line via RNAi and assessed its biological behavior changes (cell viability, migration, and invasion). The correlation between CLDN7 and epithelial-mesenchymal transition (EMT) was analyzed. Additionally, a subcutaneous tumor formation model was used to assess SACC-LM cells tumorigenicity after the CLDN7 knockdown. In the present study, we found the CLDN7 expression of tumor group was lower than that in normal salivary glands and was significantly correlated with lymph node metastasis, recurrence, and gender. CLDN7 knockdown could add the proliferation and metastasis ability of SACC by regulating EMT through Wnt/β-catenin signaling pathway. In addition, CLDN7 knockdown in SACC promoted tumor growth in nude mice. CLDN7 inhibits cell proliferation and metastasis by inactivating the Wnt/β-catenin signaling in SACC. Thus, CLDN7 expression might be a useful marker to identify the potential for progression in SACC. SAGE Publications 2020-08-04 /pmc/articles/PMC7563826/ /pubmed/32749148 http://dx.doi.org/10.1177/0963689720943583 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Cancer Pharmacogenomics and Target Therapy Ji, Huan Ding, Xu Zhang, Wei Zheng, Yang Du, Hongming Zheng, Yangyu Song, Haiyang Li, Meng Jiang, Yue Xie, Jiaxiang Wu, Ming Jiao, Pengfei Wang, Zeyu Wu, Heming Zhong, Yi Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title | Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title_full | Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title_fullStr | Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title_full_unstemmed | Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title_short | Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling |
title_sort | claudin-7 inhibits proliferation and metastasis in salivary adenoid cystic carcinoma through wnt/β-catenin signaling |
topic | Cancer Pharmacogenomics and Target Therapy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563826/ https://www.ncbi.nlm.nih.gov/pubmed/32749148 http://dx.doi.org/10.1177/0963689720943583 |
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