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Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eo...

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Autores principales: Dorosz, Aleksandra, Grosicki, Marek, Dybas, Jakub, Matuszyk, Ewelina, Rodewald, Marko, Meyer, Tobias, Popp, Jürgen, Malek, Kamilla, Baranska, Malgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563840/
https://www.ncbi.nlm.nih.gov/pubmed/32906767
http://dx.doi.org/10.3390/cells9092041
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author Dorosz, Aleksandra
Grosicki, Marek
Dybas, Jakub
Matuszyk, Ewelina
Rodewald, Marko
Meyer, Tobias
Popp, Jürgen
Malek, Kamilla
Baranska, Malgorzata
author_facet Dorosz, Aleksandra
Grosicki, Marek
Dybas, Jakub
Matuszyk, Ewelina
Rodewald, Marko
Meyer, Tobias
Popp, Jürgen
Malek, Kamilla
Baranska, Malgorzata
author_sort Dorosz, Aleksandra
collection PubMed
description Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process.
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spelling pubmed-75638402020-10-27 Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy Dorosz, Aleksandra Grosicki, Marek Dybas, Jakub Matuszyk, Ewelina Rodewald, Marko Meyer, Tobias Popp, Jürgen Malek, Kamilla Baranska, Malgorzata Cells Article Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process. MDPI 2020-09-07 /pmc/articles/PMC7563840/ /pubmed/32906767 http://dx.doi.org/10.3390/cells9092041 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dorosz, Aleksandra
Grosicki, Marek
Dybas, Jakub
Matuszyk, Ewelina
Rodewald, Marko
Meyer, Tobias
Popp, Jürgen
Malek, Kamilla
Baranska, Malgorzata
Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title_full Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title_fullStr Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title_full_unstemmed Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title_short Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
title_sort eosinophils and neutrophils—molecular differences revealed by spontaneous raman, cars and fluorescence microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563840/
https://www.ncbi.nlm.nih.gov/pubmed/32906767
http://dx.doi.org/10.3390/cells9092041
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